- All ()
- Bio Diagnostics ()
- Cells ()
- Drug Delivery & Vaccines ()
- Micro & Nano Bubbles ()
- Nanoparticles ()
- Nanopore ()
- Theranostics ()
- TRPS Technology ()
- Vesicles ()
- Viruses & Bacteria ()
- Nanopore
- qEV
- qEV (qNANO)
- qEVoriginal
- qNano
- qViro
- SEC
- Test Product
- TRPS
- Date
Extracellular Vesicles: Recent Developments in Technology and Perspectives for Cancer Liquid Biopsy
Vesicles, qNanoAbstract Extracellular micro- and nanoscale membrane vesicles produced by different cells progressively attract the attention of the scientific community. They function as mediators of intercellular communication and transport genetic...
Techniques Associated with Exosome Isolation for Biomarker Development: Liquid Biopsies for Ovarian Cancer Detection
Vesicles, qEVAbstract Ovarian cancer is the leading gynecological malignancy worldwide. This is attributed to the fact that the disease is often diagnosed at an advanced stage, where the survival rates drop ......
Personalized detection of circling exosomal PD-L1 based on Fe3O4@TiO2 isolation and SERS immunoassay
Nanoparticles, qNanoAbstract Circling exosomal PD-L1 can be expected as a predictor for the clinical responds of anti-PD-1/PD-L1 therapy. Here, we present a simple method integrating capture and analysis of exosomal PD-L1 ......
Extracellular vesicles transmit epithelial growth factor activity in the intestinal stem cell niche
Vesicles, qNanoAbstract Extracellular vesicles (EV) are membrane‐surrounded vesicles that represent a novel way of intercellular communication by carrying biologically important molecules in a concentrated and protected form. The intestinal...
MiR-21 derived from the exosomes of MSCs regulates the death and differentiation of neurons in patients with spinal cord injury
Vesicles, qNanoAbstract In this study, we aimed to investigate the therapeutic effect of miR-21 in the treatment of spinal cord injury (SCI) as well as its underlying molecular mechanisms. Real-time PCR ......
Dietary Na+ intake in healthy humans changes the urine extracellular vesicle prostasin abundance while vesicle excretion rate, NCC and ENaC are not altered
Vesicles, qNanoAbstract Low Na+ intake activates aldosterone signaling which increases renal Na+ reabsorption through increased apical activity of NaCl co-transporter (NCC) and epithelial Na+ channel (ENaC). Na+ transporter proteins are excreted ......
Enhancement of Oncolytic Activity of oHSV Expressing IL-12 and Anti PD-1 Antibody by Concurrent Administration of Exosomes Carrying CTLA-4 miRNA
Vesicles, qNanoAbstract Systemic administration of checkpoint inhibitors alone and especially concurrent with intratumoral administration of oncolytic herpesviruses (oHSV) has a major impact on cancer therapy marred by rare failures of healthy ......
Development of DNA-anchored assembly of small extracellular vesicle for efficient antigen delivery to antigen presenting cells
Vesicles, qNanoAbstract Tumor-cell derived small extracellular vesicle (sEV) combined with immunostimulatory adjuvants may serve as a promising tumor vaccine through the induction of the cytotoxic T cell response. To achieve an ......
Circulating exosomes may identify biomarkers for cows at risk for metabolic dysfunction
Vesicles, qEVAbstract Disease susceptibility of dairy cows is greatest during the transition from pregnancy to lactation. Circulating exosomes may provide biomarkers to detect at-risk cows to enhance health and productivity. From ......
Isolation and Characterization of Extracellular Vesicles from Keratinocyte Cultures
Vesicles, qEVAbstract Extracellular vesicles (EVs), including exosomes, are nano-sized membrane-bound particles which are released by cells. They have been found in all examined body fluids and can be isolated from conditioned ......
Liquid biopsies in pancreatic cancer
VesiclesABSTRACT Introduction: Pancreatic ductal adenocarcinoma (PDAC) is a disease of high lethality. Invasive tissue biopsies of primary or metastatic lesions remain the gold standard for diagnosis, but repeated sampling is ......
Acidification effects on isolation of extracellular vesicles from bovine milk
Vesicles, qEV (qNANO)Abstract Bovine milk extracellular vesicles (EVs) attract research interest as carriers of biologically active cargo including miRNA from donor to recipient cells to facilitate intercellular communication. Since toxicity of edible ......
Small Extracellular Vesicles Are Key Regulators of Non-cell Autonomous Intercellular Communication in Senescence via the Interferon Protein IFITM3
Vesicles, qEVSenescence is a cellular phenotype present in health and disease, characterized by a stable cell-cycle arrest and an inflammatory response called senescence associated secretory phenotype (SASP). The SASP is important ......
Development and comparative analysis of human urine exosome isolation strategies
VesiclesAbstract Exosomes contain virtually every type of biomolecules that have originated from parental cells. High concentrations of exosomes are found in a variety of body fluids including urine, a body ......
The Anti-Apoptotic E of ASC-Exosomes in an In Vitro ALS Model and Their Proteomic Analysis
Vesicles, qNanoAbstract Stem cell therapy represents a promising approach in the treatment of several neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). The beneficial effect of stem cells is exerted by paracrine ......
Exosome reporter mice reveal the involvement of exosomes in mediating neuron to astroglia communication in the CNS
Vesicles, qNanoAbstract Astroglia play active and diverse roles in modulating neuronal/synaptic functions in the CNS. How these astroglial functions are regulated, especially by neuronal signals, remains largely unknown. Exosomes, a major ......
Modern Techniques for the Isolation of Extracellular Vesicles and Viruses
Vesicles, qEVAbstract Extracellular signaling is pivotal to maintain organismal homeostasis. A quickly emerging field of interest within extracellular signaling is the study of extracellular vesicles (EV), which act as messaging vehicles ......
Assessment of extracellular vesicles, associated microRNAs, and of their utility as clinical biomarkers in liquid biopsies
Vesicles, qEVExtracellular vesicles (EVs) are crucial mediators of intercellular communication and promising diagnostic targets in liquid biopsies. This work aimed at assessing the applicability and utility of exploiting EVs and their ......
Acoustic immunosensing of exosomes using a quartz crystal microbalance with dissipation monitoring.
Vesicles, qEVAbstract Exosomes are endocytic lipid-membrane bound bodies with potential to be used as biomarkers in cancer and neurodegenerative disease. The limitations and scarcity of current exosome characterisation approaches has led ......
Extracellular vesicles in urologic malignancies— Implementations for future cancer care
Vesicles, qNanoAbstract Extracellular vesicles (EVs), a heterogeneous group of vesicles differing in size and shape, cargo content and function, are membrane‐bound and nano‐sized vesicles that could be released by nearly all ......
A novel population of extracellular vesicles smaller than exosomes promotes cell proliferation
Vesicles, qEVAbstract Background Extracellular vesicles (EVs) play important roles in intercellular communication by delivering RNA, lipid, and proteins to neighboring or distant cells. Identification and classification of EVs secreted from diverse ......
Exosomes derived from Induced Vascular Progenitor Cells Promote Angiogenesis in vitro and in an in vivo Rat Hindlimb Ischemia Model
Vesicles, qNanoAbstract Induced vascular progenitor cells (iVPCs) were created as an ideal cell type for regenerative medicine and have been reported to positively promote collateral blood flow and improve cardiac function ......
Tumor‐derived exosomal miRNA‐320d as a biomarker for metastatic colorectal cancer
Vesicles, qNanoAbstract Background To identify specific exosomal microRNAs (miRNAs) as serum biomarkers for prediction of metastasis in patients with colorectal cancer (CRC). Materials and Methods Serum exosomes were isolated from patients ......
MiR-126 Mediates Brain Endothelial Cell Exosome Treatment–Induced Neurorestorative Effects After Stroke in Type 2 Diabetes Mellitus Mice
Vesicles, qNanoAbstract Background and Purpose— Stroke patients with type 2 diabetes mellitus (T2DM) exhibit increased vascular and white matter damage and have worse prognosis compared with nondiabetic stroke patients. We investigated ......
The Kinetics of Small Extracellular Vesicle Delivery Impacts Skin Tissue Regeneration
Vesicles, qNanoAbstract Small extracellular vesicles (SEVs) offer a promising strategy for tissue regeneration, yet their short lifetime at the injured tissue limits their efficacy. Here, we show that kinetics of SEV ......
Macrophages Educated with Exosomes from Primed Mesenchymal Stem Cells Treat Acute Radiation Syndrome by Promoting Hematopoietic Recovery
Vesicles, qNanoABSTRACT In the setting of radiation-induced trauma, exposure to high levels of radiation can cause an acute radiation syndrome (ARS) causing bone marrow (BM) failure, leading to life-threatening infections, anemia, ......
The Role of Extracellular Vesicles and Red Blood Cell Manufacturing Methods on Transfusion-Related Immunomodulation
Vesicles, qNanoTransfusion of red blood concentrates (RCCs) is a lifesaving procedure. Nonetheless, a number of studies have revealed that transfusion of RCC products is still associated with increased risk of serious ......
Peptide Nanocarriers for the Detection of Heavy Metal Ions Using Resistive Pulse Sensing
Bio Diagnostics, qNanoAbstract The use of nanocarriers within resistive pulse sensing facilitates the detection and quantification of analytes. To date the field has been dominated by polyionic carriers or nanomaterials. Together they ......
Modification of casein aggregate microstructures under frozen conditions: a study using tunable resistive pulse sensing
Nanoparticles, qNanoAbstract Freezing and aging under frozen conditions could be a potential technique for modifying characteristics of aggregated sodium caseinate particles. In this research, 1% sodium caseinate suspensions with different NaCl ......
Transcutaneous Ultrasound-Mediated Nonviral Gene Delivery to the Liver in a Porcine Model
Drug Delivery & Vaccines, qNanoUltrasound (US)-mediated gene delivery (UMGD) of nonviral vectors was demonstrated in this study to be an effective method to transfer genes into the livers of large animals via a minimally ......
Placenta stem/stromal cell‐derived extracellular vesicles for potential use in lung repair
Vesicles, qNanoAbstract Many acute and chronic lung injuries are incurable and rank as the fourth leading cause of death globally. While stem cell treatment for lung injuries is a promising approach, ......
The Impact of Nylon-3 Copolymer Composition on the Efficiency of siRNA Delivery to Glioblastoma Cells
Nanoparticles, qNanoAbstract Glioblastoma multiforme is a devastating disease that has attracted enormous attention due to poor prognosis and high recurrence. Small interfering RNA (siRNA) in principle offers a promising therapeutic approach ......
Stem cell‐derived extracellular vesicles attenuate the early inflammatory response after tendon injury and repair
Vesicles, qNanoABSTRACT Adipose‐derived stem cells (ASCs) have the potential to enhance tendon repair via paracrine regulation of the inflammatory response to injury. Extracellular vesicles (EVs), which are secreted by ASCs, have ......
Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis
Vesicles, qEV (qNANO)Abstract The use of extracellular vesicles, specifically exosomes, as carriers of biomarkers in extracellular spaces has been well demonstrated. Despite their promising potential, the use of exosomes in the clinical ......
Neural Recovery after Cortical Injury: Effects of MSC Derived Exosomes in the Cervical Spinal Cord
Vesicles, qNanoABSTRACT Stroke is the leading cause of long-term disability costing the United States (US) health care system 34 billion dollars. However, stem cell based therapies have been shown to improve recovery after ......
Tumor-derived exosomes promote carcinogenesis of murine oral squamous cell carcinoma
Vesicles, qNanoAbstract Circulating tumor-derived exosomes (TEX) interact with a variety of cells in cancer-bearing hosts, leading to cellular reprogramming which promotes disease progression. To study TEX effects on the development of ......
Inhibition effect of exosomes-mediated Let-7a on the development and metastasis of triple negative breast cancer by down-regulating the expression of c-Myc
Vesicles, qNanoAbstract. – OBJECTIVE: Triple negative breast cancer is typically characterized by high malignancy, easy recurrence and metastasis, and poor prognosis. However, effective treatment for triple negative breast cancer has not yet been...
Conception of nanosized hybrid liposome/poloxamer particles to thicken the interior core of liposomes and delay hydrophilic drug delivery
Drug Delivery & Vaccines, qNanoAbstract Liposomes are nanocarriers composed of phospholipids, especially designed to potentially carry drugs. However, liposomes suffer in terms of leakage of small hydrophilic drugs. To control the release, a system ......
Small extracellular vesicles containing arginase-1 suppress T-cell responses and promote tumor growth in ovarian carcinoma
Vesicles, qNanoAbstract Tumor-driven immune suppression is a major barrier to successful immunotherapy in ovarian carcinomas (OvCa). Among various mechanisms responsible for immune suppression, arginase-1 (ARG1)-carrying small extracellular vesicles...
MS-Based Proteomic Analysis of Serum and Plasma: Problem of High Abundant Components and Lights and Shadows of Albumin Removal
Vesicles, qEVAbstract Blood serum or plasma proteome is a gold mine of disease biomarkers. However, complexity and a huge dynamic range of their components, combined with multiple mechanisms of degradation and ......
Increased extracellular vesicle miRNA-466 family in the bronchoalveolar lavage fluid as a precipitating factor of ARDS
Vesicles, qNanoAbstract Background Acute respiratory distress syndrome (ARDS) is a life-threatening disease; however, its treatment has not yet been fully established. The progression of ARDS is considered to be mediated by ......
Liquid biopsy in ovarian cancer: recent advances in circulating extracellular vesicle detection for early diagnosis and monitoring progression
Vesicles, TRPSAbstract The current biomarkers available in the clinic are not enough for early diagnosis or for monitoring disease progression of ovarian cancer. Liquid biopsy is a minimally invasive test and has ......
Evaluation of zeta potential of nanomaterials by electrophoretic light scattering: Fast field reversal versus Slow Field Reversal Mode
Vesicles, qNanoAbstract Zeta potential of nanomaterials designed to be used in nanomedicine is an important parameter to evaluate as it influences in vivo behaviour hence biological activity, efficacy and safety. As ......
Echographic and physical characterization of albumin-stabilized nanobubbles
Vesicles, qNanoAbstract There has been increasing interest in using nanobubbles (NBs) for ultrasound mediated drug delivery as well as for ultrasound imaging. Albumin NBs are especially attractive for its potential of ......
Extracellular vesicles in human skin: cross-talk from senescent fibroblasts to keratinocytes by miRNAs
Vesicles, qEVAbstract Extracellular vesicles (EVs) and their miRNA cargo are intercellular communicators transmitting their pleiotropic messages between different cell types, tissues and body fluids. Recently, they have been reported to contribute ......
MACROPHAGE CELL THERAPY TO TREAT ORTHOPEDIC INJURY
Vesicles, qNanoAbstract: An ex vivo generated population of tissue-specific alternatively-activated macrophages and methods of making and using such macrophages for treating orthopedic injury are provided....
Comprehensive analysis of circulating microRNAs in plasma of patients with pituitary adenomas
Vesicles, qNanoAbstract Background Circulating miRNAs in pituitary adenoma would help patient care especially in non-functioning adenoma cases as minimally invasive biomarkers of tumor recurrence and progression. Aim Our aim was to ......
Mesenchymal stem cell derived EVs mediate neuroprotection after spinal cord injury in rats via the microRNA-21-5p/FasL gene axis
Vesicles, qEVAbstract Spinal cord injury (SCI) represents a relatively common type of motor system trauma. While the SCI patient will experience varying degrees of paraplegia and quadriplegia, which severely affects their ......
Diversity and heterogeneity of extracellular RNA in human plasma
Vesicles, qEVAbstract Extracellular RNAs (exRNAs) are secreted by nearly all cell types and are now known to play multiple physiological roles. In humans, exRNA populations are found in nearly any physiological ......
PEI/NONOates-doped PLGA nanoparticles for eradicating methicillin-resistant Staphylococcus aureus biofilm in diabetic wounds via binding to the biofilm matrix
Nanoparticles, qNanoAbstract Wounds infected with methicillin-resistant Staphylococcus aureus (MRSA) biofilm represent a high risk in patients with diabetes. Nitric oxide (NO) has shown promise in dispersing biofilm and wound healing. For ......
Intracranial Non-thermal Ablation Mediated by Transcranial Focused Ultrasound and Phase-Shift Nanoemulsions
Nanoparticles, qNanoAbstract High intensity focused ultrasound (HIFU) mechanical ablation is an emerging technique for non-invasive transcranial surgery. Lesions are created by driving inertial cavitation in tissue, which requires significantly less peak ......
Basics and applications of tumor-derived extracellular vesicles
Vesicles, qEVAbstract Extracellular vesicle (EV)-mediated intercellular communication acts as a critical culprit in cancer development. The selective packaging of oncogenic molecules renders tumor-derived EVs capable of altering the tumor...
Oral keratinocyte-derived exosomes regulate proliferation of fibroblasts and epithelial cells
Vesicles, qEVAbstract Extracellular vesicles (EVs), including exosomes, are small membrane-bound particles released by cells. From a therapeutic point of view, EVs can often convey similar biological function as their parent cell. ......
Influence of Nanobubble Concentration on Blood–Brain Barrier Opening Using Focused Ultrasound Under Real-Time Acoustic Feedback Control
Micro & Nano Bubbles, qNanoAbstract Real-time acoustic feedback control based on harmonic emissions of stimulated microbubbles may serve as a way to achieve reliable blood–brain barrier (BBB) opening with focused ultrasound in the brain. ......
Oral keratinocyte-derived exosomes regulate proliferation of fibroblasts and epithelial cells
TRPS Technology, Vesicles, qEV, qNanoAbstract Extracellular vesicles (EVs), including exosomes, are small membrane-bound particles released by cells. From a therapeutic point of view, EVs can often convey similar biological function as their parent cell. ......
Ultrasensitive Immunoprofiling of Plasma Extracellular Vesicles Identifies Syndecan-1 as a Potential Tool for Minimally Invasive Diagnosis of Glioma
Vesicles, qEVAbstract Purpose: Liquid biopsy has great potential to improve the management of brain tumor patients at high risk of surgeryassociated complications. Here, the aim was to explore plasma extracellular vesicle (plEV) immunoprofiling ......
Novosphingobium sp. PP1Y as a novel source of outer membrane vesicles
Vesicles, qNanoAbstract Outer membrane vesicles (OMVs) are nanostructures of 20–200 nm diameter deriving from the surface of several Gram-negative bacteria. OMVs are emerging as shuttles involved in several mechanisms of communication ......
Exosomes Derived from Human Bone Marrow Mesenchymal Stem Cells Stimulated by Deferoxamine Accelerate Cutaneous Wound Healing by Promoting Angiogenesis
Vesicles, qNanoAbstract The exosomes are derived from mesenchymal stem cells (MSCs) and may be potentially used as an alternative for cell therapy, for treating diabetic wounds, and aid in angiogenesis. This ......
Extracellular Vesicles and Ebola Virus: A New Mechanism of Immune Evasion
Vesicles, qEVAbstract: Ebola virus (EBOV) disease can result in a range of symptoms anywhere from virtually asymptomatic to severe hemorrhagic fever during acute infection. Additionally, spans of asymptomatic persistence in recovering ......
Extracellular vesicle release from intestinal organoids is modulated by Apc mutation and other colorectal cancer progression factors
Vesicles, qNanoAbstract Extracellular vesicles (EVs) are membrane-surrounded structures that transmit biologically important molecules from the releasing to target cells, thus providing a novel intercellular communication mechanism. Since EVs carry...
Ultrasensitive detection of cancer biomarkers by nickel-based isolation of polydisperse extracellular vesicles from blood
Vesicles, qNanoAbstract Background Extracellular vesicles (EVs) are secreted membranous particles intensively studied for their potential cargo of diagnostic markers. Efficient and cost-effective isolation methods need to be established for the...
Exosome-based therapeutics: purification using semi-continuous multi-column chromatography
Vesicles, qNanoAbstract Extracellular vesicles, more particularly exosomes, are nanostructures of great medical interest. Similarly to other complex biopharmaceuticals such as virus, the purification of these nanovesicles is still a considerable...
The Impact of Physicochemical Characteristics on Therapeutic Efficacy of Anticancer Nanomaterials: A Review
TRPS Technology, NanoporeABSTRACT Cancer is a leading cause of death throughout the world which suffers from treatment failures mainly due to intensive toxicity and lack of effectiveness of conventional drugs. The application ......
Serum Exosomes from Newborn Piglets Restrict Porcine Epidemic Diarrhea Virus Infection
Vesicles, qEVAbstract Exosomes are vehicles in the body fluid that participate in many biological processes, especially immune responses. In this study, we employed comparative proteome analysis to investigate the roles of ......
Comparative analysis of exosome isolation methods using culture supernatant for optimum yield, purity and downstream applications
Vesicles, qEVAbstract Exosomes have received significant attention for their role in pathobiological processes and are being explored as a tool for disease diagnosis and management. Consequently, various isolation methods based on ......
Extracellular vesicle isolation methods: rising impact of size‐exclusion chromatography
VesiclesAbstract Extracellular vesicles (EVs) include a variety of nanosized vesicles released to the extracellular microenvironment by the vast majority of cells transferring bioactive lipids, proteins, mRNA, miRNA or non-coding RNA, ......
NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety
Vesicles, qNanoAbstract Plasma transfusion induces some transfusion related acute lung injury (TRALI) mediated through neutrophil extracellular traps (NETs). We investigated whether extracellular vesicles (EVs) present in plasma or obtained from...
A standardised protocol for the evaluation of small extracellular vesicles in plasma by imaging flow cytometry
Vesicles, qEVAbstract Flow cytometry provides robust, multi-parametric and quantitative information on single cells which also exhibits enormous potential as a tool for small particle characterisation. Small extracellular vesicle (sEV) detection by ......
Serum Exosome Isolation by Size-Exclusion Chromatography for the Discovery and Validation of Preeclampsia-Associated Biomarkers
Vesicles, qEVAbstract Exosomes are extracellular nanovesicles of complex and heterogeneous composition that are released in biofluids such as blood. The interest in the characterization of exosomal biochemistry has increased over the ......
Exosomes secreted by urine-derived stem cells improve stress urinary incontinence by promoting repair of pubococcygeus muscle injury in rats
Vesicles, qNanoAbstract Background Previous studies revealed that urine-derived stem cells (USCs) could promote myogenesis after the impairment of the sphincter muscles. However, the effects of exosomes secreted by USCs (USCs-Exo) were ......
Optimization of cell culture conditions for exosome isolation using mini-size exclusion chromatography (mini-SEC)
Vesicles, qNanoAbstract Extracellular vesicles (EVs) are emerging as a major intercellular communication system engaged in a variety of physiological and pathophysiological processes. Tumor-derived exosomes (TEX) are a subset of EVs of ......
Microbubbles for Nucleic Acid Delivery in Liver Using Mild Sonoporation
Micro & Nano Bubbles, qNanoAbstract Ultrasound-mediated gene delivery is an interesting approach, which could help in increasing gene transfer in deep tissues. Moreover, it allows for performing experiments guided by the image to determine ......
Quantitative and Specific Detection of Exosomal miRNAs for Accurate Diagnosis of Breast Cancer Using a Surface‐Enhanced Raman Scattering Sensor Based on Plasmonic Head‐Flocked Gold Nanopillars
Vesicles, qEVAbstract MicroRNAs in exosomes (exosomal miRNAs) have attracted increased attention as cancer biomarkers for early diagnosis and prognosis owing to their stability in body fluids. Since strong association exists between ......
The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens
Vesicles, qEV (qNANO)Abstract Background Robust protocols for the isolation of extracellular vesicles (EVs) from the rest of their excretory-secretory products are necessary for downstream studies and application development. The most widely used ......
Measuring particle size distribution of nanoparticle enabled medicinal products, the joint view of EUNCL and NCI-NCL. A step by step approach combining orthogonal measurements with increasing complexity
TRPS TechnologyAbstract The particle size distribution (PSD) and the stability of nanoparticles enabled medicinal products (NEP) in complex biological environments are key attributes to assess their quality, safety and efficacy. Despite ......
Circulating Extracellular Vesicles and Their miR “Barcode” Differentiate Alcohol Drinkers With Liver Injury and Those Without Liver Injury in Severe Trauma Patients
Vesicles, qEVShort Summary: Extracellular vesicles (EVs), released during tissue/cell injury, contain a “barcode” indicating specific microRNAs (miRs) that can uncover their origin. We examined whether systemic EVs possessing hepatic miR-signatures...
Article | OPEN | Published: 12 February 2019 The Regenerative Potential of Amniotic Fluid Stem Cell Extracellular Vesicles: Lessons Learned by Comparing Different Isolation Techniques
Vesicles, qEVAbstract Extracellular vesicles (EVs) derived from amniotic fluid stem cells (AFSCs) mediate anti-apoptotic, pro-angiogenic, and immune-modulatory effects in multiple disease models, such as skeletal muscle atrophy and Alport syndrome. A ......
SPRi-based hemagglutinin quantitative assay for influenza vaccine production monitoring
Viruses & Bacteria, qNanoAbstract Influenza vaccine manufacturers lack tools, whatever the involved production bioprocess (egg or cell-based), to precisely and accurately evaluate vaccine antigen content from samples. Indeed, the gold standard single-radial...
Extracellular vesicle‐educated macrophages promote early Achilles tendon healing
Vesicles, qNanoTendon healing follows a complex series of coordinated events that ultimately produces a mechanically inferior tissue more scar‐like than native tendon. More regenerative healing occurs when anti‐inflammatory M2 macrophages play ......
Surface-engineered Design of Efficient Luminescent Europium(III) Complex-based Hydroxyapatite Nanocrystals for Rapid HeLa Cancer Cell Imaging
Nanoparticles, qNanoAbstract We synthesized hydroxyapatite nanocrystals under the existence of tris(2,2,6,6-tetramethyl-3,5-heptanedionato)europium(III) (EuTH) complex to form inorganic/organic hybrid nanocrystal (EHA). Then, the folic acid derivative...
Label-free detection of exosomes using a surface plasmon resonance biosensor
Vesicles, qNanoAbstract The development of a sensitive and specific detection platform for exosomes is highly desirable as they are believed to transmit vital tumour-specific information (mRNAs, microRNAs, and proteins) to remote ......
Treponema pallidum induces the activation of endothelial cells via macrophage-derived exosomes
Vesicles, qNanoAbstract Recent studies have shown that exosomes play a role in pathogenesis and in the treatment of inflammatory diseases and tumours. We explored the effects of Treponema pallidum-induced macrophage-derived exosomes ......
Ultrasensitive immunoprofiling of plasma extracellular vesicles identifies syndecan-1 as a potential tool for minimally invasive diagnosis of glioma
Vesicles, qNanoAbstract Purpose: Liquid biopsy has great potential to improve the management of brain tumor patients at high risk of surgery-associated complications. Here, the aim was to explore plasma extracellular vesicle ......
Annexin A1 as Neuroprotective Determinant for Blood-Brain Barrier Integrity in Neonatal Hypoxic-Ischemic Encephalopathy
Vesicles, qNanoAbstract: Blood-brain barrier (BBB) disruption is associated with hypoxia-ischemia (HI) induced brain injury and life-long neurological pathologies. Treatment options are limited. Recently, we found that mesenchymal stem/stromal cell...
Breast cancer‐derived exosomes reflect the cell‐of‐origin phenotype
Vesicles, qNanoAbstract A manner in which cells can communicate with each other is via secreted nanoparticles termed exosomes. These vesicles contain lipids, nucleic acids, and proteins, and are said to reflect ......
Exosomes derived from clinical grade oral mucosal epithelial cell sheets promote wound healing
TRPS Technology, VesiclesABSTRACT The oral mucosa exhibits unique regenerative properties, sometimes referred to as foetal-like wound healing. Researchers from our institute have used sheets of oral mucosa epithelial cells (OMECs) for regenerative ......
Exosomes derived from cardiomyocytes promote cardiac fibrosis via myocyte-fibroblast cross-talk
Vesicles, qNanoAbstract Cardiac fibrosis is primarily mediated by activated fibroblasts. However, cardiomyocytes have also been implicated in facilitating the fibrotic response. We aimed to explore how cardiomyocyte-derived exosomes affect...
Characterization of Nanomaterials: Tools and Challenges
Nanoparticles, TRPSAbstract As the use and potential use of nanomaterials (NMs) in food and food-related products increases, the need for well-defined and established methods for characterization of NM properties is essential ......
Complete Disaggregation of MCF-7-derived Breast Tumour Spheroids with Very Low Concentrations of α-Mangostin Loaded in CD44 Thioaptamer-tagged Nanoparticles
Vesicles, qNanoAbstract Background: α-Mangostin (αMG) is a natural substance that exerts a wide range of antitumor effects. Recently, we described that free αMG was able to dissociate multicellular tumour spheroids (MCTSs) ......
Control of Acoustic Cavitation for Efficient Sonoporation with Phase-Shift Nanoemulsions
Bio Diagnostics, qNanoAbstract Acoustic cavitation can be used to temporarily disrupt cell membranes for intracellular delivery of large biomolecules. Termed sonoporation, the ability of this technique for efficient intracellular delivery (i.e., >50% ......
A Label-free Platform for Identification of Exosomes from Different Sources
Vesicles, qNanoAbstract Exosomes contain cell- and cell-state-specific cargos of proteins, lipids, and nucleic acids and play significant roles in cell signaling and cell–cell communication. Current research into exosome-based biomarkers has relied ......
Exosomes secreted by endothelial progenitor cells accelerate bone regeneration during distraction osteogenesis by stimulating angiogenesis
Vesicles, qNanoAbstract Background Distraction osteogenesis (DO) is an effective but lengthy procedure to fully induce bone regeneration in large bone defects. Accumulating evidence supports the role of exosomes secreted by endothelial ......
Very Long-Chain C24:1 Ceramide Is Increased in Serum Extracellular Vesicles with Aging and Can Induce Senescence in Bone-Derived Mesenchymal Stem Cells
Bio Diagnostics, qEVAbstract: Extracellular vesicles (EVs), including exosomes and microvesicles, function in cell-to-cell communication through delivery of proteins, lipids and microRNAs to target cells via endocytosis and membrane fusion. These vesicles...
Tumor-derived extracellular vesicles inhibit osteogenesis and exacerbate myeloma bone disease
Vesicles, qNanoAbstract Background: As a hallmark driver of multiple myeloma (MM), MM bone disease (MBD) is unique in that it is characterized by severely impaired osteoblast activity resulting from blocked osteogenesis ......
Serum Exosome Isolation by Size-Exclusion Chromatography for the Discovery and Validation of Preeclampsia-Associated Biomarkers
Vesicles, qEV, SECAbstract Exosomes are extracellular nanovesicles of complex and heterogeneous composition that are released in biofluids such as blood. The interest in the characterization of exosomal biochemistry has increased over the ......
Cardiac Progenitor Cell–Derived Extracellular Vesicles Reduce Infarct Size and Associate with Increased Cardiovascular Cell Proliferation
Vesicles, qNano, TRPSCell transplantation studies have shown that injection of progenitor cells can improve cardiac function after myocardial infarction (MI). Transplantation of human cardiac progenitor cells (hCPCs) results in an increased ejection ......
Red cell supernatant effects on endothelial cell function and innate immune activation is influenced by donor age and sex
Vesicles, qNano, TRPSBackground and objectives Blood donor characteristics significantly affect the pre‐storage and post‐storage quality of red cell concentrates (RCCs). This study investigated the impact of donor age and sex on RCC ......
Exosomal piRNA sequencing reveals differences between heart failure and healthy patients
Vesicles, qNanoOBJECTIVE: Heart failure is a leading cause of cardiovascular mortality in in- dustrialized countries. Increasing evidence has highlighted the relationship between noncoding regulatory RNAs, especially microRNAs, and heart failure....
Integrated Nanoscale Deterministic Lateral Displacement Arrays for Separation of Extracellular Vesicles from Clinically-Relevant Volumes of Biological Samples
Vesicles, qEVExtracellular vesicles (EVs) offer many opportunities in early-stage disease diagnosis, treatment monitoring, and precision therapy owing to their high abundance in bodily fluids, accessibility from liquid biopsy, and presence of ......
Resistive pulse sensing as particle counting and sizing method in microfluidic systems: Designs and applications review
TRPS Technology, qNano, TRPSResistive pulse sensing is a well‐known and established method for counting and sizing particles in ionic solutions. Throughout its development the technique has been expanded from detection of biological cells ......
Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells Using Microbubbles and Low Intensity Pulsed Ultrasound on 3D Printed Scaffolds
Micro & Nano Bubbles, qNano, TRPSLipid‐coated microbubbles, clinically approved as contrast enhancing agents for ultrasound imaging, are investigated for the first time for their possible applications in bone tissue engineering. Effects of microbubbles (average diameter ......
ANGI-10. WSD1227: A BRAIN PENETRABLE VEGFR2 INHIBITOR FOR THE TREATMENT OF PRIMARY AND METASTATIC BRAIN TUMORS
Vesicles, qEVThe VEGF pathway has emerged as an important target for cancer therapy by blocking the development of malignant neovasculature, thus to reduce oxygen availability to the tumor and decrease its ......
Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis
Vesicles, qEVExosomes, the smallest subset of extracellular vesicles (EVs), have recently attracted much attention in the scientific community. Their involvement in intercellular communication and molecular reprogramming of different cell types...
Mesenchymal Stem Cell-Derived Exosomes Reduce A1 Astrocytes via Downregulation of Phosphorylated NFκB P65 Subunit in Spinal Cord Injury
Vesicles, qNano, TRPSBackground/Aims: Neurotoxic A1 astrocytes are induced by inflammation after spinal cord injury (SCI), and the inflammation-related Nuclear Factor Kappa B (NFκB) pathway may be related to A1-astrocyte activation. Mesenchymal stem ......
Chromatographic Approaches for Purification and Analytical Characterization of Extracellular Vesicles: Recent Advancements
Vesicles, qEVExtracellular vesicles (EVs) are nanoscale particles released by cells under both physiological and pathological conditions, that mediate intercellular communication by delivering biomolecules (nucleic acids, proteins, and lipids). In...
Humic acids modify the pulse size distributions in the characterization of plastic microparticles by Tunable Resistive Pulse Sensing
TRPS Technology, qNano, TRPSTunable Resistive Pulse Sensing, TRPS, is an emerging technique used in quantification and measuring the size (particle-by-particle) of viruses, exosomes and engineered colloidal spheres in biological fluids. We study the ......
Generation of therapeutic cells using extracellular components of target organs
Vesicles, qNano, TRPSThe invention relates to an ex vivo generated population of tissue-specific anti-inflammatory macrophages and methods of making and using such macrophages...
EGFR detection in extracellular vesicles of breast cancer patients through immunosensor based on silica-chitosan nanoplatform
Vesicles, qEVIn the present work, we designed a microfluidic electrochemical immunosensor with enough sensibility and precision to quantify epithermal growth factor receptor (EGFR) in plasma extracellular vesicles (EVs) of plasma from ......
Extraction and liposome reconstitution of membrane proteins with their native lipids without the use of detergents
Drug Delivery & Vaccines, qNano, TRPSFunctional studies of membrane-bound channels, transporters or signal transducers require that the protein of interest resides in a membrane that separates two compartments. One approach that is commonly used to ......
Microvesicle and stem cell compositions for therapeutic applications
Vesicles, qNano, TRPSProvided herein are stem cell and exosome compositions having therapeutic utility to treat a variety of diseases and disorders, e.g., cardiovascular disease, Duchenne muscular dystrophy, and fibrotic disease....
A Comparison of Clinically Relevant Sources of Mesenchymal Stem Cell-Derived Exosomes: Bone Marrow and Amniotic Fluid
Vesicles, qNano, TRPSBackground/Purpose Exosomes may constitute a more practical alternative to live cells in select stem cell-based therapies. We sought to compare exosomes from two mesenchymal stem cell (MSC) sources relevant to ......
Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging
Vesicles, TRPSExtracellular vesicles (EVs) have prevalent roles in cancer biology and regenerative medicine. Conventional techniques for characterising EVs including electron microscopy (EM), nanoparticle tracking analysis (NTA) and tuneable...
MSC-derived exosomes ameliorate erectile dysfunction by alleviation of corpus cavernosum smooth muscle apoptosis in a rat model of cavernous nerve injury
Vesicles, qNano, TRPSBackground This study investigated the therapeutic effects of MSC-derived exosomes (MSC-Exos) on erectile function in a rat model of cavernous nerve injury (CNI). Methods MSCs were isolated from rat bone ......
Extracellular vesicle microRNA cargo is correlated with HPV‐status in oropharyngeal carcinoma
Vesicles, qNano, TRPSBackground The incidence of human papilloma virus positive (HPV+) oropharyngeal squamous cell carcinoma (OPSCC) has increased rapidly in recent decades. These tumours have a favourable outcome compared to HPV‐negative (HPV‐) ......
A Magnetic-Based Microfluidic Device for On-Chip Isolation and Detection of Tumor-Derived Exosomes
Vesicles, qNano, TRPSExosomes are membrane-enclosed phospholipid extracellular vesicles which can act as mediators of intercellular communication. Although the original features endow tumor-derived exosomes great potential as biomarkers, efficient...
Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization
Vesicles, qNano, TRPSAbstract Interest in extracellular vesicles and in particular microvesicles and exosomes, which are constitutively produced by cells, is on the rise for their huge potential as biomarkers in a high ......
Blood manufacturing methods affect red blood cell product characteristics and immunomodulatory activity
Cells, qNano, TRPSAbstract Transfusion of red cell concentrates (RCCs) is associated with increased risk of adverse outcomes that may be affected by different blood manufacturing methods and the presence of extracellular vesicles ......
Highly sensitive detection of exosomes by SERS using gold nanostar@Raman reporter@nanoshell structures modified with a bivalent cholesterol-labeled DNA anchor
Vesicles, qNano, TRPSExosomes, as important signal transmitters, play a key role in intercellular communication, especially in cancer metastasis. There is considerable evidence that exosomes can be used as an indicator of cancer. ......
A Comparison of an Optimized Ultracentrifugation Method versus Size- Exclusion Chromatography for Isolation of Exosomes from Human Serum
Vesicles, qEVExosomes are nanosized vesicles that are abundant in biological fluids. In recent years, exosomes have attracted increasing attention as their cargo may provide promising biomarkers for the early diagnosis of ......
Colorectal Cancer-derived Small Extracellular Vesicles Establish an Inflammatory Pre-metastatic Niche in Liver Metastasis
Vesicles, qNano, TRPSLiver metastases develop in more than half of the patients with colorectal cancer (CRC) and are associated with a poor prognosis. The factors influencing liver metastasis of CRC are poorly ......
Screening of red blood cells for extracellular vesicle content as a product quality indicator
Vesicles, qNano, TRPSBACKGROUND The controversy around the quality and clinical impact of stored and differentially manufactured red cell concentrates (RCCs) from different donor groups is ongoing. Current studies are limited by the ......
Ebola Virus VP40 Modulates Cell Cycle and Biogenesis of Extracellular Vesicles
Vesicles, qEVBackground Ebola virus (EBOV) mainly targets myeloid cells; however, extensive death of T cells is often observed in lethal infections. We have previously shown that EBOV VP40 in exosomes causes ......
Dopamine-loaded blood exosomes targeted to brain for better treatment of Parkinson’s disease
Vesicles, qNano, TRPSAbstract Parkinson’s disease (PD), one of the most common movement and neurodegenerative disorders, is challenging to treat, largely because the blood-brain barrier blocks passage of most drugs. Here we find ......
Gold Nanoparticles with Ligand/Zwitterion Hybrid Layer for Individual Counting of Influenza A H1N1 Subtype Using Resistive Pulse Sensing
Nanoparticles, TRPS Technology, qNano, TRPSResistive pulse sensing (RPS) is an analytical technique for detecting particles with nano- to micrometer diameters, such as proteins, viruses, and bacteria. RPS is a promising tool for diagnosis as ......
Provocative decompression causes diffuse vascular injury in mice mediated by microparticles containing interleukin-1β
Vesicles, qNano, TRPSInflammatory mediators are known to be elevated in association with decompression from elevated ambient pressure, but their role in tissue damage or overt decompression sickness is unclear. Circulating microparticles (MPs) ......
Urinary exosome miR‐146a is a potential marker of albuminuria in essential hypertension
Vesicles, qNano, TRPSBackground There is increasing interest in using extracellular vesicle-derived microRNAs (miRNAs) as biomarkers in renal dysfunction and injury. Preliminary evidence indicates that miRNAs regulate the progression of glomerular disease....
Biophysical virus particle specific characterization to sharpen the definition of virus stability
Viruses & Bacteria, qNano, TRPSVaccine thermostability is key to successful global immunization programs as it may have a significant impact on the continuous cold-chain maintenance logistics, as well as affect vaccine potency. Modern biological ......
Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine
Vesicles, qEVExtracellular vesicles (EVs) have a great potential in clinical applications. However, their isolation from different bodily fluids and their characterisation are currently not optimal or standardised. Here, we report the ......
Bioprocess Decision Support Tool For Scalable Manufacture of Extracellular Vesicles
Vesicles, qEVNewly recognized as natural nanocarriers that deliver biological information between cells, extracellular vesicles (EVs), including exosomes and microvesicles, provide unprecedented therapeutic opportunities. Large‐scale and...
Zhang, Suisui, Huijun Dai, Lingyu Zhu, Fei Lin, Zhaokun Hu, Ren Jing, Weikang Zhang et al. “Microvesicles packaging IL-1β and TNF-α enhance lung inflammatory response to mechanical ventilation in part by induction of cofilin signaling.” International immunopharmacology 63 (2018): 74-83.
Vesicles, qNano, TRPSMicrovesicles shed from pulmonary cells are capable of transferring inflammatory cargo to recipient cells nearby or in distant to enhance inflammation. Some authors believe that cofilin controls actin dynamics and ......
Bioinspired, nanoscale approaches in contemporary bioanalytics
TRPS Technology, qNano, TRPSThe genesis for this topical review stems from the interdisciplinary Biointerfaces International conference 2016 (BI 2016) in Zurich, Switzerland, wherein the need for advances in analytical tools was both expressed ......
Probing the conformational switch of I-motif DNA using tunable resistive pulse sensing
TRPS Technology, qNano, TRPSI-motif DNA, which can fold and unfold reversibly in various environments, plays a significant role in DNA nanotechnology and biological functions. Thus, it is of fundamental importance to identify the ......
Loss of XIST in Breast Cancer Activates MSN-c-Met and Reprograms Microglia via Exosomal miRNA to Promote Brain Metastasis
Vesicles, qEVUp to 30% of patients with metastatic breast cancer eventually develop brain metastasis, yet the pathologic mechanism behind this development remains poorly understood. Here, we profiled long noncoding RNAs in ......
Multipotent mesenchymal stromal cell–derived exosomes improve functional recovery after experimental intracerebral hemorrhage in the rat
Vesicles, qNano, TRPSOBJECTIVE Previous studies have demonstrated that transplanted multipotent mesenchymal stromal cells (MSCs) improve functional recovery in rats after experimental intracerebral hemorrhage (ICH). In this study the authors tested the...
Delivery of MGMT mRNA to glioma cells by reactive astrocyte-derived exosomes confers a temozolomide resistance phenotype
Vesicles, qNano, TRPSThe glioma-astrocyte interaction plays an important role in tumor microenvironment remodeling; however, the underlying mechanism has not been completely clarified. In this study, we show that glioma cells stimulate normal ......
Tissue and exosomal miRNA editing in Non-Small Cell Lung Cancer
Vesicles, qEVRNA editing in microRNAs has been recently proposed as a novel biomarker in cancer. Here, we investigated RNA editing by leveraging small-RNA sequencing data from 87 NSCLC (Non-Small Cell Lung ......
Strategic design of extracellular vesicle drug delivery systems
Vesicles, qEVExtracellular vesicles (EVs), sub-micron vectors used in intercellular communication, have demonstrated great promise as natural drug delivery systems. Recent reports have detailed impressive in vivo results from the administration of ......
Circulating extracellular vesicles are associated with lipid and insulin metabolism
Vesicles, qEV, SECWe have reported that hypertrophic adipocytes release extracellular vesicles (EVs) and that number of circulating adipocyte-derived EVs correlated with insulin and homeostasis model assessment-insulin resistance (HOMA-IR) in a pilot...
Carcinoma-associated fibroblasts promote the stemness and chemoresistance of colorectal cancer by transferring exosomal lncRNA H19
Theranostics, Vesicles, qNano, TRPSLong non-coding RNAs (lncRNAs) are involved in the pathology of various tumors, including colorectal cancer (CRC). The crosstalk between carcinoma- associated fibroblasts (CAFs) and cancer cells in the tumor microenvironment ......
In Vivo Evaluation of Magnetic Targeting in Mice Colon Tumors with Ultra-Magnetic Liposomes Monitored by MRI
Drug Delivery & Vaccines, qNano, TRPSPurpose The development of theranostic nanocarriers as an innovative therapy against cancer has been improved by targeting properties in order to optimize the drug delivery to safely achieve its desired ......
N-(2-Hydroxypropyl)methacrylamide polymer conjugated pyropheophorbidea, a promising tumor-targeted theranostic probe for photodynamic therapy and imaging
Vesicles, qNano, TRPSTumor-targeted photodynamic therapy (PDT) using polymeric photosensitizers is a promising therapeutic strategy for cancer treatment. In this study, we synthesized a pHPMA conjugated pyropheophorbide-a (P-PyF) as a cancer theranostic...
Methods and Technologies for Exosome Isolation and Characterization
Vesicles, qNano, TRPSAbstract Exosomes, a specific subclass of the extracellular vesicles secreted by most cell types, play an important role in cell–cell communication by transporting diverse content including protein, mRNA, miRNA, and ......
Quantification of Exosome Based on Copper-Mediated Signal Amplification Strategy
Vesicles, qNano, TRPSExosomes, a class of small extracellular vesicles, play important roles in various physiological and pathological processes by serving as vehicles for transferring and delivering membrane and cytosolic molecules between cells. ......
Alzheimer’s disease pathology propagation by exosomes containing toxic amyloid‐beta oligomers
Vesicles, qNano, TRPSThe gradual deterioration of cognitive functions in Alzheimer’s disease is paralleled by a hierarchical progression of amyloid-beta and tau brain pathology. Recent findings indicate that toxic oligomers of amyloid-beta may ......
Systematic Methodological Evaluation of a Multiplex Bead-Based Flow Cytometry Assay for Detection of Extracellular Vesicle Surface Signatures
Vesicles, qEVExtracellular vesicles (EVs) can be harvested from cell culture supernatants and from all body fluids. EVs can be conceptually classified based on their size and biogenesis as exosomes and microvesicles. ......
mRNA transcript distribution bias between Borrelia burgdorferi bacteria and their outer membrane vesicles
Vesicles, qEVBorrelia burgdorferi, a bacterium in the spirochete phylum, is the causative agent of Lyme disease. Borrelia burgdorferi has a linear chromosome with a number of circular and linear plasmids. Bacteria, ......
Low cardiorespiratory fitness is associated with higher extracellular vesicle counts in obese adults
Vesicles, qNano, TRPSAbstract Low cardiorespiratory fitness (CRF) is associated with cardiovascular disease (CVD) independent of obesity. Extracellular vesicles (EVs) are a novel target of CVD, however, it remains unknown if obese individuals ......
Novel analytical methods to assess the chemical and physical properties of liposomes
Drug Delivery & Vaccines, qNano, TRPSLiposomes are used in commercial pharmaceutical formulations (PFs) and dietary supplements (DSs) as a carrier vehicle to protect the active ingredient from degradation and to increase the half-life of the ......
High-throughput screening identified selective inhibitors of exosome biogenesis and secretion: A drug repurposing strategy for advanced cancer
Vesicles, qNano, TRPSTargeting exosome biogenesis and release may have potential clinical implications for cancer therapy. Herein, we have optimized a quantitative high throughput screen (qHTS) assay to identify compounds that modulate exosome ......
Low cardiorespiratory fitness is associated with higher extracellular vesicle counts in obese adults
Vesicles, qNano, TRPSAbstract Low cardiorespiratory fitness (CRF) is associated with cardiovascular disease (CVD) independent of obesity. Extracellular vesicles (EVs) are a novel target of CVD, however, it remains unknown if obese individuals ......
Novel analytical methods to assess the chemical and physical properties of liposomes
Drug Delivery & Vaccines, qNano, TRPSLiposomes are used in commercial pharmaceutical formulations (PFs) and dietary supplements (DSs) as a carrier vehicle to protect the active ingredient from degradation and to increase the half-life of the ......
Dendri-tac and their use as theranostics
Theranostics, qNano, TRPSThe present invention relates to novel amphiphilic dendrimers, hereafter denoted Dendri-TAC. The present invention also relates to perfluorocarbon nanoemulsions stabilized by these amphiphilic dendrimers and their uses for in vivo ......
Diagnostic and Therapeutic Applications of Exosomes in Cancer
Vesicles, qEV, qNano, TRPSDiagnostic and Therapeutic Applications of Exosomes in Cancer evaluates the potential of exosome content manipulation in the development of novel therapeutics. In recent years, exosomes, the small vesicles produced by ......
Microvesicles from Human Adipose Tissue-Derived Mesenchymal Stem Cells as a New Protective Strategy in Osteoarthritic Chondrocytes
Vesicles, qNano, TRPSAbstract Background/Aims: Chronic inflammation contributes to cartilage degeneration during the progression of osteoarthritis (OA). Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and...
Origins-to-Outcomes-A-Role-for-Extracellular-Vesicles-in-Precision-Medicine
Vesicles, qEV, SECExtracellular vesicles (EVs) are of great interest in biological research, and though they are a relatively recent discovery, they have rapidly shown great potential for use in clinical applications. The ......
Microvesicles from Human Adipose Tissue- Derived Mesenchymal Stem Cells as a New Protective Strategy in Osteoarthritic Chondrocytes
Vesicles, qNano, TRPSAbstract Background/Aims: Chronic inflammation contributes to cartilage degeneration during the progression of osteoarthritis (OA). Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and...
The Design and Characterisation of Multifunctional Aptamer Nanopore Sensors
Bio Diagnostics, qNano, TRPSAptamer-modified nanomaterials provide a simple, yet powerful sensing platform when combined with resistive pulse sensing technologies. Aptamers adopt a more stable tertiary structure in the presence of a target analyte, ......
Transmembrane NADH Oxidation with Tetracyanoquinodimethane
Nanoparticles, qNano, TRPSThe design of efficient schemes for nicotinamide adenine dinucleotide (NAD) regeneration is essential for the development of enzymatic biotechnological processes in order to sustain continuous production. In line with our ......
Mesenchymal Stem Cell-Derived Exosomes Provide Neuroprotection and Improve Long-Term Neurologic Outcomes in a Swine Model of Traumatic Brain Injury and Hemorrhagic Shock
Vesicles, qNano, TRPSCombined traumatic brain injury (TBI) and hemorrhagic shock (HS) remains a leading cause of preventable death worldwide. Mesenchymal stem cell-derived exosomes have demonstrated promise in small animal models of neurologic ......
Proteome profiling of exosomes derived from plasma of heifers with divergent genetic merit for fertility
Vesicles, qEV, SECThe current study evaluated exosomes isolated from plasma of heifers bred to have high or low fertility through developing extreme diversity in fertility breeding values, however, key animal traits (e.g., ......
The impact of circulating preeclampsia-associated extracellular vesicles on the migratory activity and phenotype of THP-1 monocytic cells
Vesicles, qEVIntercellular communication via extracellular vesicles (EVs) and their target cells, especially immune cells, results in functional and phenotype changes that consequently may play a significant role in various physiological states ......
None of us is the same as all of us: resolving the heterogeneity of extracellular vesicles using single-vesicle, nanoscale characterization with resonance enhanced atomic force microscope infrared spectroscopy (AFM-IR)
Vesicles, qEVThe successful translation of nanoparticle-based systems into commercial products depends upon the ability to reliably measure important physical and chemical properties of these particles. The density of nanoparticles is one ......
Measuring the size and density of nanoparticles by centrifugal sedimentation and flotation
Nanoparticles, qNano, TRPSThe successful translation of nanoparticle-based systems into commercial products depends upon the ability to reliably measure important physical and chemical properties of these particles. The density of nanoparticles is one ......
Intra-Cardiac Release of Extracellular Vesicles Shapes Inflammation Following Myocardial Infarction
Vesicles, qNano, TRPSAbstract Rationale: A rapid and massive influx of inflammatory cells occurs into ischemic area following myocardial infarction, resulting in local release of cytokines and growth factors. Yet, the mechanisms regulating ......
Measuring the size and density of nanoparticles by centrifugal sedimentation and flotation
Nanoparticles, qNano, TRPSThe successful translation of nanoparticle-based systems into commercial products depends upon the ability to reliably measure important physical and chemical properties of these particles. The density of nanoparticles is one ......
Tumor-associated macrophages-derived exosomes promote the migration of gastric cancer cells by transfer of functional Apolipoprotein E
Vesicles, qNano, TRPSTumor-associated macrophages (TAMs) are a major component of the tumor microenvironment and have been shown to contribute to tumor aggressiveness. However, the detailed mechanisms underlying the pro-metastatic effect of TAMs ......
Intra-Cardiac Release of Extracellular Vesicles Shapes Inflammation Following Myocardial Infarction
Vesicles, qNano, TRPSAbstract Rationale: A rapid and massive influx of inflammatory cells occurs into ischemic area following myocardial infarction, resulting in local release of cytokines and growth factors. Yet, the mechanisms regulating ......
Detection and proteomic characterization of extracellular vesicles in human pancreatic juice
Vesicles, qNano, TRPSAbstract Aims The prognosis of patients with pancreatic cancer has remained virtually unchanged with a high mortality rate compared to other types of cancers. An earlier detection would provide a ......
Characteristics of Extracellular Vesicles in Red Blood Concentrates Change with Storage Time and Blood Manufacturing Method
Vesicles, qNano, TRPSKeywords Microvesicles · Microparticles · Red blood cells · In vitro quality · Storage lesion · Blood processing · Red cell filtered · Whole blood filtered Summary Background: Extracellular vesicles (EVs) ......
Circulating extracellular vesicles are associated with lipid and insulin metabolism
Vesicles, qEVAbstract We have reported that hypertrophic adipocytes release extracellular vesicles (EVs) and the number of circulating adipocyte-derived EVs correlated with insulin and homeostasis model assessment-insulin resistance (HOMA-IR) in a...
METASTASIS AND ADAPTIVE RESISTANCE INHIBITING IMMUNOTHERAPY COMBINED ONLINECHEMOTHERAPY (43)Pub.Date: Dec.13,2018 WITH RADIOTHERAPY’STUMOR SEEKING EXTRACELLULAR VESICLESWITH SIRNA AND CHEMOTHERAPEUTICS
Vesicles, qEVAbstract Mutated genome silencing with endogenous RNAi-siRNA and miRNA with near total cellular apheresis with pulse flow apheresis system and EV-exosome-RNA molecular apheresis with sucrose density gradient continuous flow...
3D visualization of extracellular vesicle uptake by endothelial cells
Vesicles, qNanoAbstract Background Extracellular vesicles are small vesicles that contain cytoplasmic and membrane components from their paternal cells. They enter target cells through uptake to transfer their biological cargo. In this ......
Extracellular Vesicles from Amnion-Derived Mesenchymal Stem Cells Ameliorate Hepatic Inflammation and Fibrosis in Rats
Vesicles, qNanoAbstract Background. There are no approved drug treatments for liver fibrosis and nonalcoholic steatohepatitis (NASH), an advanced stage of fibrosis which has rapidly become a major cause of cirrhosis. Therefore, ......
Facile Functionalization of Gold Nanoparticles with PLGA Polymer Brushes and Efficient Encapsulation into PLGA Nanoparticles: Toward Spatially Precise Bioimaging of Polymeric Nanoparticles
Nanoparticles, qNanoAbstract Nanocarriers prepared from poly(lactide‐co‐glycolide) (PLGA) have broad biomedical applications. Understanding their cellular uptake and distribution requires appropriate visualization in complex biological compartments with...
The Role of Arrestin Domain-Containing 3 in Regulating Endocytic Recycling and Extracellular Vesicle Sorting of Integrin β4 in Breast Cancer
Vesicles, qEVDespite the established role of integrin β4 (ITG β4) in breast cancer progression, the importance of endocytic recycling of ITG β4 and its regulatory mechanism are poorly understood. Here, we ......
Metastasis-associated miR-23a from nasopharyngeal carcinomaderived exosomes mediates angiogenesis by repressing a novel target gene TSGA10
Vesicles, qEVBenefiting from more precise imaging and radiotherapy, patients with locoregionally nasopharyngeal carcinoma (NPC) have a significantly higher survival rate. Nonetheless, distant metastasis is still the predominant mode of failure....
Extracellular vesicle RNAs reflect placenta dysfunction and are a biomarker source for preterm labour
Vesicles, qEVPreterm birth (PTB) can lead to lifelong complications and challenges. Identifying and monitoring molecular signals in easily accessible biological samples that can diagnose or predict the risk of preterm labour ......
Enhancing clot properties through fibrin-specific self-cross-linked PEG side-chain microgels
Drug Delivery & Vaccines, qNano, TRPSExcessive bleeding and resulting complications are a major cause of death in both trauma and surgical settings. Recently, there have been a number of investigations into the design of synthetic ......
miRNAs targeting ICP4 and delivered to susceptible cells in exosomes block herpes simplex virus 1 replication in dose dependent manner
Vesicles, qNano, TRPSmiRNAs are potent tools that in principle can be used to control the replication of infectious agents. The objectives of the studies reported here were to design miRNAs that can ......
Bead Enhancement of EV Analysis
Vesicles, qEVExtracellular vesicles (EVs) are recognized cancer biomarkers, however, clinical analysis has been difficult due to a lack of simple and sensitive assays. Here, we describe a bead-enhanced flow cytometry method, ......
Separation of plasma-derived exosomes into CD3(+) and CD3(-) fractions allows for association of immune cell and tumor cell markers with disease activity in HNSCC patients
Vesicles, qNano, TRPSHead and neck squamous cell carcinoma (HNSCC) is a highly immunosuppressive malignancy. Exosomes in HNSCC patients’ plasma are enriched in inhibitory cargo and mediate immunosuppression. As these exosomes are products ......
Nano-/Microparticle Tracers for Evaluating Structures in Fractured Porous Media
Nanoparticles, qNano, TRPSNano- and microparticle tracers are expected to be used for evaluating structures in geological developments, however their transport behavior is not well characterized. This study investigated nanoparticle transport in a ......
Extracellular vesicles have variable dose-dependent effects on cultured draining cells in the eye
Vesicles, qNano, TRPSThe role of extracellular vesicles (EVs) as signal mediators has been described in many biological fields. How many EVs are needed to deliver the desired physiological signal is yet unclear. ......
Extracellular vesicles as modulators of wound healing
Vesicles, qNano, TRPSImpaired healing of cutaneous wounds and ulcers continues to have a major impact on the quality of life of millions of people. In recent years, the capacity for stem and ......
Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles
Vesicles, qEVUnderstanding the role of extracellular RNA (exRNA) has emerged as an exciting avenue for biomarker, therapeutic, as well as basic cell–cell communication applications and discoveries. Multiple protocols, kits, and procedures ......
Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression Using NanoString
Vesicles, qEVSeveral different types of RNA molecules such as microRNAs (miRNAs) have been detected within extracellular vesicles in the circulation. The detection and potential utility of these as disease biomarkers requires ......
Technical challenges of working with extracellular vesicles
Vesicles, qNano, TRPSExtracellular vesicles (EVs) are small membrane vesicles secreted from cells and have great potential as drug delivery carriers. Surface proteins on EV membranes might play roles in pharmacokinetics. One method ......
Technical challenges of working with extracellular vesicles
Vesicles, qEV, qNano, TRPSExtracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit...
Exosomal miRNAs as novel cancer biomarkers: Challenges and opportunities
Vesicles, qEV, SECA biomarker with high specificity and sensitivity, is a basic requirement for non-invasive cancer diagnosis. Exosomes are a type of lipid bilayer extracellular vesicles (EVs), containing different components, including proteins, ......
Engineered nanointerfaces for microfluidic isolation and molecular profiling of tumor-specific extracellular vesicles
Vesicles, qNano, TRPSExtracellular vesicles (EVs) carry RNA, DNA, proteins, and lipids. Specifically, tumor-derived EVs have the potential to be utilized as disease-specific biomarkers. However, a lack of methods to isolate tumor-specific EVs ......
Comparison of membrane afnity-based method with size-exclusion chromatography for isolation of exosome-like vesicles from human plasma
Vesicles, qEV, SECBackground Plasma extracellular vesicles (EVs), especially exosome-like vesicles (ELVs), are being increasingly explored as a source of potential noninvasive disease biomarkers. The discovery of blood-based biomarkers associated with...
On-a-chip tryptic digestion of transthyretin: a step toward an integrated microfluidic system for the follow-up of familial transthyretin amyloidosis
Nanoparticles, TRPS Technology, qNano, TRPSA microfluidic microreactor for trypsin mediated transthyretin (TTR) digestion has been developed as a step towards the elaboration of a fully integrated microdevice for the detection of a rare and ......
A Multiparameter Approach to Separation and Clonal Analysis of Mammalian Cells
Vesicles, qNano, TRPSMammalian cell bioprocessing is a critical part of both diagnostic and therapeutic applications. In terms of diagnostics, blood based biomarkers can be isolated from the peripheral blood and analyzed to ......
Direct and label-free influenza virus detection based on multisite binding to sialic acid receptors
Drug Delivery & Vaccines, qNano, TRPSA system to discriminate human or avian influenza A remains a highly sought-after tool for prevention of influenza pandemics in humans. Selective binding of the influenza A viral hemagglutinin (HA) ......
Imaging flow cytometry for the characterization of extracellular vesicles
TRPS Technology, qNano, TRPSExtracellular Vesicles (EVs) are potent bio-activators and inter-cellular communicators that play an important role in both health and disease. It is for this reason there is a strong interest in ......
Cationic amphiphile in phospholipid bilayer or oil–water interface of nanocarriers affects planktonic and biofilm bacteria killing
Bio Diagnostics, Drug Delivery & Vaccines, Micro & Nano Bubbles, qNano, TRPSA cationic amphiphile, soyaethyl morpholinium ethosulfate (SME), immobilized in liposomes or nanoemulsions, was prepared in an attempt to compare the antibacterial activity between SME intercalated in the phospholipid bilayer and ......
Multiplex Fluorescent Immunoassay Device Based on Magnetic Nanoparticles
Nanoparticles, qNano, TRPSImmunofluorescent analyzer based compact disc for simultaneous detection of 3 antibiotics in the same milk sample is consisting of two parts: CD-based immunofluorescence kit and optoelectronic fluorometer. Kit consists of ......
Single molecule characterisation of metal nanoparticles using nanopore-based stochastic detection methods
Nanoparticles, qNano, TRPSSingle-molecule techniques are revolutionising analytical chemistry methods by observing one molecule at a time, the specific dynamics of populations can be revealed, and the averaging of signals from many molecules, ......
Creating aptamers and their use in Resisitive Pulse Sensors
TRPS Technology, qNano, TRPSResistive pulse sensors, RPS, are allowing the transport mechanism of molecules, proteins and even nanoparticles to be characterized as they traverse small channels. Here we present our recent advancement of ......
THE REGULATION OF ROTAVIRUS–INFECTED HT29.F8 AND MA104 CELLS TREATED WITH A CHIDIN 1 OR A CHIDIN 3
Viruses & Bacteria, qEV, qNano, TRPSRotavirus (RV) infections cause severe life threatening diarrhea in young children and immunocompromised individuals. Several effective vaccines have been developed for young children but are not protective against all strains ......
Novel method for the formation of monodisperse superheated perfluorocarbon nanodroplets as activatable ultrasound contrast agents
Bio Diagnostics, Micro & Nano Bubbles, qNano, TRPSMicrobubble (MB) contrast agents have positively impacted the clinical ultrasound (US) community worldwide. Their use in molecular US imaging applications has been hindered by their limited distribution to the vascular ......
Synthesis and characterization of noble metal–titania core–shell nanostructures with tunable shell thickness
Nanoparticles, qNano, TRPSCore–shell nanostructures have found applications in many fields, including surface enhanced spectroscopy, catalysis and solar cells. Titania-coated noble metal nanoparticles, which combine the surface plasmon resonance properties of...
Integrated Method for Purification and Single-Particle Characterization of Lentiviral Vector Systems by Size Exclusion Chromatography and Tunable Resistive Pulse Sensing
TRPS Technology, Viruses & Bacteria, qEV, qNano, TRPSElements derived from lentiviral particles such as viral vectors or virus-like particles are commonly used for biotechnological and biomedical applications, for example in mammalian protein expression, gene delivery or therapy, ......
Larger or more? Nanoparticle characterisation methods for recognition of dimers
TRPS Technology, qNano, TRPSOur article dissects the problem of understanding the origin of size heterogeneity in polydispersed nanoparticle samples. A commercially available multimodal material representing a typical borderline case of the nano definition ......
Biological Functions and Current Advances in Isolation and Detection Strategies for Exosome Nanovesicles
Vesicles, qNano, TRPSExosomes are nanoscale (≈30–150 nm) extracellular vesicles of endocytic origin that are shed by most types of cells and circulate in bodily fluids. Exosomes carry a specific composition of proteins, ......
Why the need and how to approach the functional diversity of extracellular vesicles
Vesicles, qNano, TRPSAbstract In the past decade, cell-to-cell communication mediated by exosomes has attracted growing attention from biomedical scientists and physicians, leading to several recent publications in top-tier journals. Exosomes are generally ......
Exacerbation of Acute Traumatic Brain Injury by Circulating Extracellular Vesicles
Vesicles, qNano, TRPSInflammatory lesions in the brain activate a systemic acute phase response (APR), which is dependent on the release of extracellular vesicles (EVs) into the circulation. The resulting APR is responsible ......
Immunomodulatory role for membrane vesicles released by THP-1 macrophages and respiratory pathogens during macrophage infection
Vesicles, qNano, TRPSBackground During infection, inflammation is partially driven by the release of mediators which facilitate intercellular communication. Amongst these mediators are small membrane vesicles (MVs) that can be released by both ......
Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies
Vesicles, qEV, qNano, SEC, TRPSAppropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease. We hypothesized that ultrafiltration followed by size exclusion ......
GMSC-Derived Exosomes Combined with a Chitosan/Silk Hydrogel Sponge Accelerates Wound Healing in a Diabetic Rat Skin Defect Model
Vesicles, qEV, qNano, SEC, TRPSBackground: Delayed wound healing in diabetic patients is one of the most challenging complications in clinical medicine, as it poses a greater risk of gangrene, amputation and even death. Therefore, ......
Notochordal-cell derived extracellular vesicles exert regenerative effects on canine and human nucleus pulposus cells
Vesicles, qEV, SECDuring intervertebral disc ageing, chondrocyte-like cells (CLCs) replace notochordal cells (NCs). NCs have been shown to induce regenerative effects in CLCs. Since vesicles released by NCs may be responsible for ......
Exosome Isolation: Is There an Optimal Method with Regard to Diagnosis or Treatment?
Vesicles, qEV, SECExtracellular vesicles (EV) gained considerable interest in recent years as both diagnostic tools and templates for therapeutic applications. EVs carry a number of cell-specific markers which gave researchers the opportunity ......
Budesonide, fluticasone propionate, and azithromycin do not modulate the membrane vesicle release by THP-1 macrophages and respiratory pathogens during macrophage infection
Vesicles, Viruses & Bacteria, qNano, TRPSPatients with more severe chronic obstructive pulmonary disease frequently experience exacerbations and it is estimated that up to 50% of these exacerbations are associated with bacterial infections. The mainstay treatment ......
Adipose-derived circulating miRNAs regulate gene expression in other tissues
Vesicles, qNano, TRPSAdipose tissue is a major site of energy storage and has a role in the regulation of metabolism through the release of adipokines. Here we show that mice with an ......
Identification and characterization of serovar-independent immunogens in Actinobacillus pleuropneumoniae
Vesicles, Viruses & Bacteria, qNano, TRPSDespite numerous actions to prevent disease, Actinobacillus pleuropneumoniae (A. pleuropneumoniae) remains a major cause of porcine pleuropneumonia, resulting in economic losses to the swine industry worldwide. In this paper, we ......
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper
Vesicles, SECThe release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. ......
Toward Exosome-Based Therapeutics: Isolation, Heterogeneity, and Fit-for-Purpose Potency
Vesicles, qNano, TRPSExosomes are defined as submicron (30–150 nm), lipid bilayer-enclosed extracellular vesicles (EVs), specifically generated by the late endosomal compartment through fusion of multivesicular bodies with the plasma membrane. Produced by ......
Exosomal microRNA signatures in multiple sclerosis reflect disease status
Vesicles, qEV, SECMultiple Sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system (CNS). There is currently no single definitive test for MS. Circulating exosomes represent promising candidate biomarkers ......
Quantitative real-time single particle analysis of virions
Viruses & Bacteria, qViro, TRPSProviding information about single virus particles has for a long time been mainly the domain of electron microscopy. More recently, technologies have been developed-or adapted from other fields, such as ......
Monitoring bacterial growth using tunable resistive pulse sensing with a pore-based technique
Viruses & Bacteria, qNano, TRPSA novel bacterial growth monitoring method using a tunable resistive pulse sensor (TRPS) system is introduced in this study for accurate and sensitive measurement of cell size and cell concentration ......
Isolation of extracellular vesicles: General methodologies and latest trends
Vesicles, qNano, TRPSBackground Extracellular vesicles (EVs) play an essential role in the transport of diagnostically significant molecules and communication between adjacent and distant cells within the body. Numerous methods for isolation of ......
Exosomes derived from cardiac telocytes exert positive effects on endothelial cells
Vesicles, qNano, TRPSTelocytes are novel cells that have been documented in the interstitium of multiple organs; however, their role in the heart remains unclear. This study aimed to identify cardiac telocytes by ......
Challenges in Analysis of Circulating Extracellular Vesicles in Human Plasma Using Nanotracking and Tunable Resistive Pulse Sensing
TRPS Technology, Vesicles, qNano, TRPSAim: Extracellular vesicles (EV) are secreted from both healthy and diseased tissues and are detectable in most body uids, where their measurement can be of prognostic/diagnostic value. We aimed to ......
Sorting of small infectious virus particles by flow virometry reveals distinct infectivity profiles
qViro, TRPSThe nature and concentration of lipids and proteins at the surface of viruses are essential parameters for determining particle infectiveness. Historically, averaged bulk analysis of viral particles has been the ......
Extracellular vesicles as emerging targets in cancer: Recent development from bench to bedside
Vesicles, qEVExtracellular vesicles (EVs) have emerged as important players of cancer initiation and progression through cell-cell communication. They have been recognized as critical mediators of extracellular communications, which promote...
A method for the isolation and enrichment of purified bovine milk exosomes
Vesicles, qEVExosomes are nanovesicles that play important roles in intercellular communication as they carry information to target cells. Isolation of high purity exosomes will aid in studying the exosomal cargo and quantity as ......
Isolation of Exosomes for the Purpose of Protein Cargo Analysis with the Use of Mass Spectrometry
Vesicles, qNano, TRPSExosomes are intercellular messengers with a high potential for diagnostic and therapeutic utility. It is believed that exosomes present in body fluids are responsible for providing signals which inhibit immune ......
Extracellular Vesicles: Evolutionarily Conserved Mediators of Intercellular Communication
Vesicles, qNano, TRPSExtracellular vesicles (EV) are sub-micron circulating vesicles found in all bodily fluids and in all species so far tested. They have also recently been identified in seawater and it has ......
Extracellular vesicles from adipose-derived mesenchymal stem cells down-regulate senescence features in osteoarthritic osteoblasts
Vesicles, qNano, TRPSAbstract Osteoarthritis (OA) affects all articular tissues leading to pain and disability. The dysregulation of bone metabolism may contribute to the progression of this condition. Adipose-derived mesenchymal stem cells (ASC) ......
High-purified Isolation and Proteomic Analysis of Urinary Exosomes from Healthy Persons
Vesicles, qNano, TRPSUrinary exosomes containing specific biomarkers have recently been considered as novel potential non-invasive candidates for renal disease diagnosis. However, the development of urinary exosomes in basic research and their subsequent ......
Exosomes of human placenta-derived mesenchymal stem cells stimulate angiogenesis
Vesicles, qNano, TRPSBackground The therapeutic potential of mesenchymal stem cells (MSCs) may be attributed partly to humoral factors such as growth factors, cytokines, and chemokines. Human term placental tissue-derived MSCs (PlaMSCs), or ......
Effect of exosomes from plasma of dairy cows with or without an infected uterus on prostaglandin production by endometrial cell lines
Vesicles, qEVA contributing factor to declining fertility in dairy cows is an activated inflammatory system associated with uterine infection. Detecting uterine disease using biomarkers may allow earlier diagnosis and intervention with ......
Selective release of miRNAs via extracellular vesicles is associated with house dust mite allergen-induced airway inflammation
Vesicles, qNano, TRPSBackground MicroRNAs (miRNAs) may facilitate cell-to-cell communication via extracellular vesicles (EVs). The biological roles of miRNAs in EVs on allergic airway inflammation is unclear. Methods Airway secreted EVs (AEVs) were ......
Identification of exosomal muscle-specific miRNAs in serum of myotonic dystrophy patients relating to muscle disease progressIdentification of exosomal muscle-specific miRNAs in serum of myotonic dystrophy patients relating to muscle disease progress
Vesicles, qNano, TRPSMyotonic dystrophy type 1 (DM1) is the most common form of adult-onset muscular dystrophy, which is characterised by progressive muscle wasting and the discovery of reliable blood-based biomarkers could be ......
Exosomes secreted from mutant-HIF-1α-modified bone-marrow-derived mesenchymal stem cells attenuate early steroid-induced avascular necrosis of femoral head in rabbit
Vesicles, qNano, TRPSMesenchymal stem cells (MSCs)-derived exosomes exhibit protective effects on damaged or diseased tissues. Hypoxia-inducible factor 1α (HIF-1α) plays a critical role in bone development. However, HIF-1α is easily biodegradable under ......
Exosomes Derived from Mesenchymal Stem Cells Rescue Myocardial Ischaemia/Reperfusion Injury by Inducing Cardiomyocyte Autophagy Via AMPK and Akt Pathways
Vesicles, qNano, TRPSBackground/Aims: Reperfusion after an ischaemic insult might cause infarct extension. Mesenchymal stem cell (MSC)-derived exosomes could attenuate myocardial remodelling in animal models of myocardial ischaemia reperfusion injury...
Mesenteric lymph node stromal cell-derived extracellular vesicles contribute to peripheral de novo induction of Foxp3+ regulatory T cells
Vesicles, qNano, TRPSIntestinal regulatory T cells (Tregs) are fundamental in peripheral tolerance towards commensals and food-borne antigens. Accordingly, gut-draining mesenteric lymph nodes (mLNs) represent a site of efficient peripheral de novo Treg...
Involvement of Interleukin-34 in the Communications between Osteosarcoma and their Microenvironment
Vesicles, qNano, TRPSInterleukin-34 (IL-34) is a cytokine which binds and activates the M-CSF receptor (M- CSFR). Consequently, IL-34 shares functional similarities with M-CSF such as the regulation of the myeloid cell differentiation. ......
Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA
Vesicles, qNano, TRPSRecently, biological roles of extracellular vesicles (which include among others exosomes, microvesicles and apoptotic bodies) have attracted substantial attention in various fields of biomedicine. Here we investigated the impact of ......
Biogenesis of Pro-senescent Microparticles by Endothelial Colony Forming Cells from Premature Neonates is driven by SIRT1-Dependent Epigenetic Regulation of MKK6
Vesicles, qNano, TRPSSenescent cells may exert detrimental effect on microenvironment through the secretion of soluble factors and the release of extracellular vesicles, such as microparticles, key actors in ageing and cardiovascular diseases. ......
Isolation of Biologically Active Exosomes from Plasma of Patients with Cancer
Vesicles, qNano, TRPSA method for exosome isolation from human plasma was developed for rapid, high-throughput processing of plasma specimens obtained from patients with cancer. This method removes the bulk of plasma proteins ......
Current knowledge on exosome biogenesis and release
Vesicles, qNano, TRPSExosomes are nanosized membrane vesicles released by fusion of an organelle of the endocytic pathway, the multivesicular body, with the plasma membrane. This process was discovered more than 30 years ago, ......
Oncogenic transformation of lung cells results in distinct exosome protein profile similar to the cell of origin
Vesicles, qEV, qNano, TRPSLung cancer is responsible for the highest rate of cancer mortality worldwide. Lung cancer patients are often ineligible for tumour biopsies due to co-morbidities. As a result, patients may not ......
Exosome Isolation: Is There an Optimal Method with Regard to Diagnosis or Treatment?
Vesicles, qEVExtracellular vesicles (EV) gained considerable interest in recent years as both diagnos‐ tic tools and templates for therapeutic applications. EVs carry a number of cell-specific markers which gave researchers the ......
Mining Extracellular Vesicles for Clinically Relevant Noninvasive Diagnostic Biomarkers in Cancer
Vesicles, qNano, TRPSExtracellular vesicles (EVs) are nanosized vesicles secreted by virtually all cell types into the extracellular milieu. EVs transport bioactive molecules between cells and play multifaceted roles in cell-to-cell communications and ......
Exosomes as biomarkers and therapeutic tools for type 1 diabetes mellitus
Vesicles, qNano, TRPSEarly diagnosis of diabetes mellitus can significantly improve therapeutic strategies and overall health span. Identifying biomarkers as a tool for determining the risk of developing diabetes as well as a ......
Extracellular vesicles are independent metabolic units with asparaginase activity
Vesicles, qNano, TRPSExtracellular vesicles (EVs) are membrane particles involved in the exchange of a broad range of bioactive molecules between cells and the microenvironment. Although it has been shown that cells can ......
Identification of Exosomal Muscle-Specific miRNAs in Serum of Myotonic Dystrophy Patients Relating to Muscle Disease Progress
Vesicles, qNano, TRPSMyotonic dystrophy type 1 (DM1) is the most common form of adult-onset muscular dystrophy, which is characterised by progressive muscle wasting and the discovery of reliable blood-based biomarkers could be ......
Bead-based flow-cytometry for semi-quantitative analysis of complex membrane vesicle populations released by bacteria and host cells
Vesicles, qNano, TRPSDuring infection, the release of nano-sized membrane vesicle is a process which is common both for bacteria and host cells. Host cell-derived membrane vesicles can be involved in innate and ......
The Methods of Choice for Extracellular Vesicles (EVs) Characterization
Vesicles, TRPSIn recent years, extracellular vesicles (EVs) have become a subject of intense study. These membrane-enclosed spherical structures are secreted by almost every cell type and are engaged in the transport ......
Quantum dot-based sensitive detection of disease specific exosome in serum
Vesicles, qNano, TRPSTumor-derived exosomes have emerged as promising cancer biomarkers due to their unique composition and functions. Herein, we report a stripping voltammetric immunoassay for the electrochemical detection of tumor-specific exosomes using ......
Cigarette smoke extract induced exosome release is mediated by depletion of exofacial thiols and can be inhibited by thiol-antioxidants
Vesicles, qNano, TRPSIntroduction Airway epithelial cells have been described to release extracellular vesicles (EVs) with pathological properties when exposed to cigarette smoke extract (CSE). As CSE causes oxidative stress, we investigated whether ......
Augmented liver targeting of exosomes by surface modification with cationized pullulan
Vesicles, qNano, TRPSExosomes are membrane nanoparticles containing biological substances that are employed as therapeutics in experimental inflammatory models. Surface modification of exosomes for better tissue targetability and enhancement of their...
Isolation of Exosomes from HTLV-Infected Cells
Vesicles, qNano, TRPSExosomes are small vesicles, approximately 30–100 nm in diameter, that transport various cargos, such as proteins and nucleic acids, between cells. It has been previously shown that exosomes can also ......
Exosomes derived from mesenchymal non‐small cell lung cancer cells promote chemoresistance
Vesicles, qNano, TRPSNon-small cell lung cancer (NSCLC) is the most common lung cancer type and the most common cause of mortality in lung cancer patients. NSCLC is often associated with resistance to ......
Exosomes: a new horizon in lung cancer
Vesicles, qEVCirculating exosomes are the major mediators of cell–cell communication. They have been found in various body fluids of healthy individuals and patients with malignancies as cargos of several molecules including ......
Comparison of exosomes secreted by induced pluripotent stem cell-derived mesenchymal stem cells and synovial membrane-derived mesenchymal stem cells for the treatment of osteoarthritis
Vesicles, qNano, TRPSBackground Osteoarthritis (OA) is the most common joint disease worldwide. In the past decade, mesenchymal stem cells (MSCs) have been used widely for the treatment of OA. A potential mechanism ......
Integration of Stem Cell-Derived Exosomes with In Situ Hydrogel Glue as a Promising Tissue Patch for Articular Cartilage Regeneration
Vesicles, qNano, TRPSThe regeneration of articular cartilage, which scarcely shows innate self-healing ability, is a great challenge in clinical treatment. Stem cell-derived exosomes (SC-Exos), an important type of extracellular nanovesicle, exhibit great ......
Extracellular Vesicle Characteristics in Stored Red Blood Cell Concentrates Are Influenced by the Method of Detection
Vesicles, qNano, TRPSExtracellular vesicles (EVs), including microvesicles and exosomes, are small phospholipid vesicles (≤1 μm in diameter) that are present in blood products, accumulate during storage, and have a potential transfusion-related...
A microRNA signature in circulating exosomes is superior to exosomal glypican-1 levels for diagnosing pancreatic cancer
Vesicles, qNano, TRPSPancreatic ductal adenocarcinoma (PDAC) is a deadly malignancy that often presents clinically at an advanced stage and that may be confused with chronic pancreatitis (CP). Conversely, CP may be misdiagnosed ......
Exosomes as Novel Bio-carriers for Gene and Drug Delivery
Vesicles, qEVClinical treatments have stalled in certain diseases due to a lack of proper therapeutic delivery systems. Recent studies have identified exosomes for their potential use as cell-free therapies, which may ......
Exosomes Secreted from Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Prevent Osteonecrosis of the Femoral Head by Promoting Angiogenesis
Vesicles, qNano, TRPSBackground: Local ischemia is the main pathological performance in osteonecrosis of the femoral head (ONFH). There is currently no effective therapy to promote angiogenesis in the femoral head. Recent studies ......
MYC mediates large oncosome-induced fibroblast reprogramming in prostate cancer
Vesicles, qNano, TRPSCommunication between cancer cells and the tumor microenvironment results in the modulation of complex signaling networks that facilitate tumor progression. Here we describe a new mechanism of intercellular communication originating ......
Mycobacterium tuberculosis Membrane Vesicles Inhibit T Cell Activation
Vesicles, qEVMycobacterium tuberculosis utilizes multiple mechanisms to evade host immune responses, and inhibition of effector CD4+ T cell responses by M. tuberculosis may contribute to immune evasion. TCR signaling is inhibited ......
Identification of potential saliva and tear biomarkers in primary Sjögren’s syndrome, utilising the extraction of extracellular vesicles and proteomics analysis
Vesicles, qEVBackground There is a long-lasting need for non-invasive, more accurate diagnostic techniques when evaluating primary Sjögren’s syndrome (pSS) patients. Incorporation of additional diagnostics involving screening for disease-specific...
Exosomal miR-146a contributes to the enhanced therapeutic efficacy of IL-1β-primed mesenchymal stem cells against sepsis
Vesicles, qNano, TRPSImproving the immunomodulatory efficacy of mesenchymal stem cells (MSCs) through pretreatment with pro-inflammatory cytokines is an evolving field of investigation. However, the underlying mechanisms have not been fully clarified. Here, ......
Exosomes in Cancer Diagnostics
Vesicles, qNano, TRPSExosomes are endosome derived extracellular vesicles of 30–120 nm size ranges. Exosomes have been identified as mediators of cell-to-cell communication by transferring bioactive molecules such as nucleic acids, proteins and ......
Detection of tissue factor-positive extracellular vesicles by laser scanning confocal microscopy
VesiclesIntroduction Increased levels of tissue factor-positive extracellular vesicles (TF + EVs) have been detected in the plasma of patients with various diseases, including cancer and endotoxemia. Levels of TF + ......
Lysozyme association with circulating RNA, extracellular vesicles, and chronic stress
Vesicles, qEVBackground Stress has demonstrated effects on inflammation though underlying cell-cell communication mechanisms remain unclear. We hypothesize that circulating RNAs and extracellular vesicles (EVs) in patients with chronic stress...
Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles
Vesicles, qNano, TRPSAccurate characterization of extracellular vesicles (EVs), including exosomes and microvesicles, is essential to obtain further knowledge on the biological relevance of EVs. Tunable resistive pulse sensing (tRPS) has shown promise ......
Diverse impact of xeno-free conditions on biological and regenerative properties of hUC-MSCs and their extracellular vesicles
Vesicles, qNano, TRPSGrowing evidence indicates that intracellular signaling mediated by extracellular vesicles (EVs) released by stem cells plays a considerable role in triggering the regenerative program upon transplantation. EVs from umbilical cord ......
Role of OmpA2 surface regions of Porphyromonas gingivalis in host–pathogen interactions with oral epithelial cells
Vesicles, qNano, TRPSOuter membrane protein A (OmpA) is a key outer membrane protein found in Gram-negative bacteria that contributes to several crucial processes in bacterial virulence. In Porphyromonas gingivalis, OmpA is predicted ......
Antigen-loaded pH-sensitive hydrogel microparticles are taken up by dendritic cells with no requirement for targeting antibodies
Drug Delivery & Vaccines, Nanoparticles, Vesicles, qNano, TRPSParticle-based delivery of encapsulated antigens has great potential for improving vaccine constructs. In this study, we show that antigen-loaded, pH-sensitive hydrogel microparticles are taken up and presented by bone marrow-derived ......
Analysis of bacteria-derived outer membrane vesicles using tunable resistive pulse sensing
Vesicles, Viruses & Bacteria, qNano, TRPSAccurate characterization of submicron particles within biological fluids presents a major challenge for a wide range of biomedical research. Detection, characterization and classification are difficult due to the presence of ......
High-Resolution Single Particle Zeta Potential Characterisation of Biological Nanoparticles using Tunable Resistive Pulse Sensing
TRPS TechnologyPhysicochemical properties of nanoparticles, such as size, shape, surface charge, density, and porosity play a central role in biological interactions and hence accurate determination of these characteristics is of utmost ......
Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation
Vesicles, qNano, TRPSUltraviolet (UV) irradiation induces skin pigmentation, which relies on the intercellular crosstalk of melanin between melanocytes to keratinocytes. However, studying the separate effects of UVA and UVB irradiation reveals differences ......
Optimized production of HIV-1 virus-like particles by transient transfection in CAP-T cells
Viruses & Bacteria, qNano, TRPSHIV-1 virus-like particles (VLPs) have great potential as new-generation vaccines. The novel CAP-T cell line is used for the first time to produce Gag-GFP HIV-1 VLPs by means of polyethylenimine ......
High transport efficiency of nanoparticles through a total-consumption sample introduction system and its beneficial application for particle size evaluation in single-particle ICP-MS
TRPS Technology, qNano, TRPSIn order to facilitate reliable and efficient determination of both the particle number concentration (PNC) and the size of nanoparticles (NPs) by single-particle ICP-MS (spICP-MS) without the need to correct ......
A modular approach towards drug delivery vehicles using oxanorbornane-based non-ionic amphiphiles
Drug Delivery & Vaccines, qNano, TRPSThe self-assembly of non-ionic amphiphiles with a hydroxylated oxanorbornane head-group was controlled using amino acid units as spacers between hydrophilic and lipophilic domains to get spherical supramolecular aggregates. The ability ......
Could microparticles be the universal quality indicator for platelet viability and function?
TRPS Technology, qNano, TRPSHigh quality means good fitness for the intended use. Research activity regarding quality measures for platelet transfusions has focused on platelet storage and platelet storage lesion. Thus, platelet quality is ......
Sepia ink as a surrogate for colloid transport tests in porous media
Nanoparticles, qNano, TRPSWe examined the suitability of the ink of Sepia officinalis as a surrogate for transport studies of microorganisms and microparticles in porous media. Sepia ink is an organic pigment consisted ......
Continuous Micro-/Nanofluidic Devices for Single-Cell Analysis
Bio Diagnostics, qNano, TRPSMicro- and nanofluidic devices are revolutionizing the fields of single-cell analysis, and benefiting related efforts in life science research, agricultural industry, and clinical medicine. These miniaturized devices introduce much...
Formulation and advantages of furazolidone in liposomal drug delivery systems
Drug Delivery & Vaccines, qNano, TRPSFurazolidone has proven to have antiprotozoal and antibacterial activity. A number of literature supported its use against Helicobacter pylori. This potential application opens new prospects of its use in clinical ......
Design of functional cationic microgels as conjugation scaffolds
Bio Diagnostics, TRPSWe present the development and detailed characterization of a range of amine functionalized microgels for utilization in conjugation reactions. Cationic N-isopropylmethacrylamide (NIPMAm) based microgels were synthesized through...
Characterisation of furazolidone-chitosan based spray dried microparticles regarding their drug release and mucin adsorptive properties
Drug Delivery & Vaccines, qNano, TRPSThe present study aimed to prepare mucoadhesive microparticles of furazolidone (antimicrobial agent) using chitosan as a mucin adsorptive polymer and spray drying technology. This study recognized the potential use of ......
Developing a Predictable Regulatory Path for Nanomedicines by Accurate and Objective Particle Measurement
Drug Delivery & Vaccines, qNano, TRPSThe approval of medicines and medical devices is well regulated worldwide, with significant literature, guidelines and legislation to ensure that appropriate procedures are adhered to in order to give confidence ......
Lipid Coated Microbubbles and Low Intensity Pulsed Ultrasound Enhance Chondrogenesis of Human Mesenchymal Stem Cells in 3D Printed Scaffolds
Micro & Nano Bubbles, qNano, TRPSLipid-coated microbubbles are used to enhance ultrasound imaging and drug delivery. Here we apply these microbubbles along with low intensity pulsed ultrasound (LIPUS) for the first time to enhance proliferation ......
Characterisation of the protein corona using tunable resistive pulse sensing: determining the change and distribution of a particle’s surface charge
Bio Diagnostics, TRPS Technology, qNano, TRPSThe zeta potential of the protein corona around carboxyl particles has been measured using tunable resistive pulse sensing (TRPS). A simple and rapid assay for characterising zeta potentials within buffer, ......
Size and ζ-Potential Measurement of Silica Nanoparticles in Serum Using Tunable Resistive Pulse Sensing
TRPS Technology, qNano, TRPSThe contact of nanoparticles with biological fluids such as serum results in rapid adsorption of proteins at the nanoparticle surface in a layer known as the “protein corona”. Protein coatings ......
Multimodal Dispersion of Nanoparticles: A Comprehensive Evaluation of Size Distribution with 9 Size Measurement Methods
Nanoparticles, TRPS Technology, qNano, TRPSPurpose Evaluation of particle size distribution (PSD) of multimodal dispersion of nanoparticles is a difficult task due to inherent limitations of size measurement methods. The present work reports the evaluation ......
Particle-by-Particle Charge Analysis of DNA-Modified Nanoparticles Using Tunable Resistive Pulse Sensing
TRPS Technology, qNano, TRPSResistive pulse sensors, RPS, are allowing the transport mechanism of molecules, proteins and even nanoparticles to be characterized as they traverse pores. Previous work using RPS has shown that the ......
Size-Exclusion Chromatography- based isolation minimally alters Extracellular Vesicles’ characteristics compared to precipitating agents
Vesicles, SECExtracellular vesicles (EVs) have become an attractive field among the scientific community. Yet, a major challenge is to define a consensus method for EVs isolation. Ultracentrifugation has been the most ......
Multivesicular body formation enhancement and exosome release during endoplasmic reticulum stress
Vesicles, qNano, TRPSThe endoplasmic reticulum (ER) plays a pivotal role in maintaining cellular homeostasis. However, numerous environmental and genetic factors give rise to ER stress by inducing an accumulation of unfolded proteins. ......
Microfluidic approaches for isolation, detection, and characterization of extracellular vesicles: Current status and future directions
Vesicles, qNano, TRPSExtracellular vesicles (EVs) are cell-derived vesicles present in body fluids that play an essential role in various cellular processes, such as intercellular communication, inflammation, cellular homeostasis, survival, transport, and...
Exosomes from iPSCs Delivering siRNA Attenuate Intracellular Adhesion Molecule-1 Expression and Neutrophils Adhesion in Pulmonary Microvascular Endothelial Cellsression and Neutrophils Adhesion in Pulmonary Microvascular Endothelial Cells
Vesicles, qNano, TRPSThe pro-inflammatory activation of pulmonary microvascular endothelial cells resulting in continuous expression of cellular adhesion molecules, and subsequently recruiting primed neutrophils to form a firm neutrophils-endothelium...
Immunosuppressive effect of mesenchymal stem cell-derived exosomes on a concanavalin A-induced liver injury model
Vesicles, qNano, TRPSBackground This study aimed to evaluate the effect of mesenchymal stem cell (MSC)-derived exosomes on an immune-induced liver injury model. MSCs show a unique function to modulate immune reaction although ......
Exosomes Derived from Human Endothelial Progenitor Cells Accelerate Cutaneous Wound Healing by Promoting Angiogenesis Through Erk1/2 Signaling
Vesicles, qNano, TRPSChronic skin wounds represent one of the most common and disabling complications of diabetes. Endothelial progenitor cells (EPCs) are precursors of endothelial cells and can enhance diabetic wound repair by ......
Digital Detection of Exosomes by Interferometric Imaging
Vesicles, qNano, TRPSExosomes, which are membranous nanovesicles, are actively released by cells and have been attributed to roles in cell-cell communication, cancer metastasis, and early disease diagnostics. The small size (30–100 nm) along ......
Plasma vesicle miRNAs for therapy response monitoring in Hodgkin lymphoma patients
TRPS Technology, Vesicles, qNano, TRPSBACKGROUND. Cell-free circulating nucleic acids, including 22-nt microRNAs (miRNAs), represent noninvasive biomarkers for treatment response monitoring of cancer patients. While the majority of plasma miRNA is bound to proteins, a ......
Urinary Extracellular Vesicles: Potential Biomarkers of Renal Function in Diabetic Patients
Vesicles, qNano, TRPSThe aim of this study was to check the relationship between the density of urinary EVs, their size distribution and the progress of early renal damage in type 2 diabetic ......
Interspecies Communication between Pathogens and Immune Cells via Bacterial Membrane Vesicles
Vesicles, qEV, qNano, TRPSThe production of extracellular vesicles is a universal mechanism for intercellular communication that is conserved across kingdoms. Prokaryotes secrete 50–250 nm membrane vesicles (MVs) in a manner that is regulated ......
Macrophages redirect phagocytosis by non- professional phagocytes and influence inflammation
Vesicles, qNano, TRPSProfessional phagocytes (such as macrophages1) and non-professional phagocytes2, 3, 4, 5, 6, 7, 8(such as epithelial cells) clear billions of apoptotic cells and particles on a daily basis9. Although professional ......
Trehalose prevents aggregation of exosomes and cryodamage
Vesicles, qNano, TRPSExosomes are important mediators in intercellular communication. Released by many cell types, they transport proteins, lipids, and nucleic acids to distant recipient cells and contribute to important physiopathological processes....
Exosome-based tumor antigenseadjuvant co-delivery utilizing genetically engineered tumor cell-derived exosomes with immunostimulatory CpG DNA
Vesicles, qNano, TRPSFor cancer immunotherapy via tumor antigen vaccination in combination with an adjuvant, major challenges include the identification of a particular tumor antigen and efficient delivery of the antigen as well ......
A Standardized Method To Determine the Concentration of Extracellular Vesicles using Tunable Resistive Pulse Sensing
TRPS Technology, Vesicles, qEV, qNano, TRPSBackground: Understanding the pathogenic role of extracellular vesicles (EVs) in disease and their potential diagnostic and therapeutic utility is extremely reliant on in-depth quantification, measurement and identification of EV...
Modulation of paracrine signaling by CD9 positive small extracellular vesicles mediates cellular growth of androgen deprived prostate cancer
Vesicles, qNano, TRPSProliferation and maintenance of both normal and prostate cancer (PCa) cells is highly regulated by steroid hormones, particularly androgens, and the extracellular environment. Herein, we identify the secretion of CD9 ......
The role of extracellular vesicle and tunneling nanotube-mediated intercellular cross-talk between mesenchymal stem cells and human peripheral T cells
Vesicles, qNano, TRPSUltraviolet (UV) irradiation induces skin pigmentation, which relies on the intercellular crosstalk of melanin between melanocytes to keratinocytes. However, studying the separate effects of UVA and UVB irradiation reveals differences ......
Delivery of membrane proteins into small and giant unilamellar vesicles by charge-mediated fusion
Vesicles, qNano, TRPSOne of the current challenges in synthetic biology is the production of stable membrane mimetic systems and the insertion of components in these systems. Here, we employ fusion of oppositely ......
Low-density lipoprotein mimics blood plasma-derived exosomes and microvesicles during isolation and detection
Vesicles, qEV, qNano, TRPSCirculating extracellular vesicles have emerged as potential new biomarkers in a wide variety of diseases. Despite the increasing interest, their isolation and purification from body fluids remains challenging. Here we ......
Exosomes Secreted by Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Repair Critical-Sized Bone Defects through Enhanced Angiogenesis and Osteogenesis in Osteoporotic Rats
Vesicles, qNano, TRPSBone defects caused by trauma, severe infection, tumor resection and skeletal abnormalities are common osteoporotic conditions and major challenges in orthopedic surgery, and there is still no effective solution to ......
Preanalytical, analytical, and biological variation of blood plasma submicron particle levels measured with nanoparticle tracking analysis and tunable resistive pulse sensing
Vesicles, qNano, TRPSBackground: Nanoparticle tracking analysis (NTA) and tunable resistive pulse sensing (TRPS) enable measurement of extracellular vesicles (EVs) in blood plasma but also measure other particles present in plasma. Complete isolation ......
Isolation of biologically active and morphologically intact exosomes from plasma of patients with cancer.
Vesicles, qNano, TRPSObjective: Isolation from human plasma of exosomes that retain functional and morphological integrity for probing their protein, lipid and nucleic acid content is a priority for the future use of ......
Extracellular vesicles isolated from brains of rTg4510 mice seed tau aggregation in a threshold-dependent manner
Vesicles, qNano, TRPSThe microtubule-associated protein tau has a critical role in Alzheimer’s disease and related tauopathies. There is accumulating evidence that tau aggregates spread and replicate in a prion-like manner, with the ......
Comparative Analysis of Technologies for Quantifying Extracellular Vesicles (EVs) in Clinical Cerebrospinal Fluids (CSF)
Vesicles, qNano, TRPSExtracellular vesicles (EVs) have emerged as a promising biomarker platform for glioblastoma patients. However, the optimal method for quantitative assessment of EVs in clinical bio-fluid remains a point of contention. ......
Extracellular vesicles in cardiovascular disease: are they Jedi or Sith?
Vesicles, qEV, qNano, TRPSIn the recent past, extracellular vesicles have become recognized as important players in cell biology and biomedicine. Extracellular vesicles, including exosomes, microvesicles and apoptotic bodies, are phospholipid bilayer-enclosed...
Cardiovascular Progenitor-Derived Extracellular Vesicles Recapitulate the Beneficial Effects of their Parent Cells in the Treatment of Chronic Heart Failure
Vesicles, qNano, TRPSBackground Cell-based therapies are being explored as a therapeutic option for patients with chronic heart failure following myocardial infarction. Extracellular vesicles (EV), including exosomes and microparticles, secreted by...
Mimicking respiratory phosphorylation using purified enzymes
Vesicles, qNano, TRPSThe enzymes of oxidative phosphorylation is a striking example of the functional association of multiple enzyme complexes, working together to form ATP from cellular reducing equivalents. These complexes, such as ......
A Modular Approach Towards Drug Delivery Vehicles Using Oxanorbornane-based Non-ionic Amphiphiles
Drug Delivery & Vaccines, qNanoAbstract The self-assembly of non-ionic amphiphiles with a hydroxylated oxanorbornane head-group was controlled using amino acid units as spacers between hydrophilic and lipophilic domains to get spherical supramolecular aggregates. The ......
Size-exclusion chromatography as a stand-alone methodology identifies novel markers in mass spectrometry analyses of plasma-derived vesicles from healthy individuals
Vesicles, qNano, TRPSPlasma-derived vesicles hold a promising potential for use in biomedical applications. Two major challenges, however, hinder their implementation into translational tools: (a) the incomplete characterization of the protein composition of ......
Dexamethasone phosphate-loaded folate-conjugated polymeric nanoparticles for selective delivery to activated macrophages and suppression of inflammatory responses
Drug Delivery & Vaccines, Nanoparticles, TRPSActivated macrophages play a central role in the pathology of inflammatory diseases by secreting pro-inflammatory cytokines. In this study, we have developed folate-conjugated dexamethasone phosphate (DP)-loaded...
Nitric oxide-releasing poly(lactic-co-glycolic acid)-polyethylenimine nanoparticles for prolonged nitric oxide release, antibacterial efficacy, and in vivo wound healing activity.
Drug Delivery & Vaccines, Nanoparticles, qNano, TRPSNitric oxide (NO)-releasing nanoparticles (NPs) have emerged as a wound healing enhancer and a novel antibacterial agent that can circumvent antibiotic resistance. However, the NO release from NPs over extended ......
Characterizations of nanospheres and nanorods using resistive-pulse sensing
Nanoparticles, TRPSNanoparticles, such as colloids, liposomes, and extracellular vesicles, have important roles in a wide range of processes and applications. The detection and characterization of nanoparticles remain technological hurdles because of ......
Characterization of vesicular stomatitis virus populations by tunable resistive pulse sensing
Viruses & Bacteria, qNano, TRPSAlthough transmission electron microscopy (TEM) has historically been the method of choice to estimate concentrations of virus and virus-like particles, these measures can often be time-consuming and labor-intensive to perform. ......
Synthesis and Characterization of Hybrid Polymer/Lipid Expansile Nanoparticles: Imparting Surface Functionality for Targeting and Stability
Drug Delivery & Vaccines, Nanoparticles, TRPSThe size, drug loading, drug release kinetics, localization, biodistribution, and stability of a given polymeric nanoparticle (NP) system depend on the composition of the NP core as well as its ......
Linking exposures of particles released from nano-enabled products to toxicology: An integrated methodology for particle sampling, extraction, dispersion and dosing
Cells, qNano, TRPSNano-enabled products (NEPs) represent a growing economic global market that integrates nanotechnology into our everyday lives. Increased consumer use and disposal of NEPs at their end of life has led ......
Vibration-assisted optical injection of a single fluorescent sensor into a target cell
Cells, TRPSIn this paper, we propose the selective adhesion and rapid injection of a fluorescent sensor into a target cell via the optical control of zeta potential and local vibration stimulus ......
Nanofiltration to remove microparticles and decrease the thrombogenicity of plasma: in vitro feasibility assessment
Cells, TRPSBACKGROUND As plasma contains procoagulant microparticles (MPs), removing MPs by 75-nm nanofiltration may decrease plasma in vitro thrombogenicity while maintaining the hemostatic activity from coagulation factors. STUDY DESIGN AND...
Acoustic Cavitation-Mediated Delivery of Small Interfering Ribonucleic Acids with Phase-Shift Nano-emulsions
Cells, qNano, TRPSLocalized, targeted delivery of small interfering ribonucleic acid (siRNA) has been the foremost hurdle in the use of siRNA for the treatment of various diseases. Major advances have been achieved ......
Dexamethasone phosphate-loaded folate-conjugated polymeric nanoparticles for selective delivery to activated macrophages and suppression of inflammatory responses
Drug Delivery & Vaccines, Nanoparticles, TRPSActivated macrophages play a central role in the pathology of inflammatory diseases by secreting pro-inflammatory cytokines. In this study, we have developed folate-conjugated dexamethasone phosphate (DP)-loaded...
Nitric oxide-releasing poly(lactic-co-glycolic acid)-polyethylenimine nanoparticles for prolonged nitric oxide release, antibacterial efficacy, and in vivo wound healing activity.
Drug Delivery & Vaccines, Nanoparticles, qNano, TRPSNitric oxide (NO)-releasing nanoparticles (NPs) have emerged as a wound healing enhancer and a novel antibacterial agent that can circumvent antibiotic resistance. However, the NO release from NPs over extended ......
Cryo-Electron Microscopy Structure of Human Peroxiredoxin-3 Filament Reveals the Assembly of a Putative Chaperone
Cells, qNano, TRPSPeroxiredoxins (Prxs) are a ubiquitous class of thiol-dependent peroxidases that play an important role in the protection and response of cells to oxidative stress. The catalytic unit of typical 2-Cys ......
Biocompatible Polymer–Peptide Hybrid-Based DNA Nanoparticles for Gene Delivery
Cells, Nanoparticles, TRPSCurrently, research on polymers to be used as gene delivery systems is one of the most important directions in both polymer science and biomedicine. In this report, we describe a ......
Nanoparticle-stabilized microbubbles for multimodal imaging and drug delivery
Drug Delivery & Vaccines, Nanoparticles, qNano, TRPSMicrobubbles (MBs) are routinely used as contrast agents for ultrasound imaging. The use of ultrasound in combination with MBs has also attracted attention as a method to enhance drug delivery. ......
Ultrasoft, highly deformable microgels
Drug Delivery & Vaccines, Nanoparticles, TRPS Technology, qNano, TRPSMicrogels are colloidally stable, hydrogel microparticles that have previously been used in a range of (soft) material applications due to their tunable mechanical and chemical properties. Most commonly, thermo and ......
Monitoring of Microalgae Lipid Accumulation System Overview
Cells, TRPS Technology, TRPSVarious methods have been proposed to monitor and determine microalgae lipid content accumulation, but they are indirect, time consuming, require sample preparations, and costly treatment steps. In this work, a ......
Conductive and Biphasic Pulses in Tunable Resistive Pulse Sensing
Nanoparticles, TRPS Technology, TRPSThis experimental study concerns the occurrence of biphasic pulses generated during tunable resistive pulse sensing (TRPS) of 200 nm carboxylate polystyrene spheres. In TRPS, a short-lived pulse in ionic current ......
Multiplexed microfluidic quantification of proteins in serum
Bio Diagnostics, TRPS Technology, TRPSRapid and low cost immunoassays targeting proteins in blood or other bodily fluids are highly sought after for point-of-care devices and early screening of patients. Immunoturbidimetric assays utilize latex particles ......
Sorting of small infectious virus particles by flow virometry reveals distinct infectivity profiles
Bio Diagnostics, TRPS Technology, Viruses & Bacteria, qNano, TRPSThe nature and concentration of lipids and proteins at the surface of viruses are essential parameters for determining particle infectiveness. Historically, averaged bulk analysis of viral particles has been the ......
Perfluorocarbon nanodroplets stabilized by fluorinated surfactants: characterization and potentiality as theranostic agent
Drug Delivery & Vaccines, Micro & Nano Bubbles, TRPS Technology, qNano, TRPSWe aim to produce emulsions that can act as contrast agents and drug carriers for cancer imaging and therapy. To increase tumor detection and decrease drug side effects, it is ......
Cationic additives in nanosystems activate cytotoxicity and inflammatory response of human neutrophils: lipid nanoparticles versus polymeric nanoparticles
Bio Diagnostics, Nanoparticles, qNano, TRPSThis report compares the effect of lipid and polymeric nanoparticles upon human neutrophils in the presence of cationic surfactants. Nanostructured lipid carriers and poly(lactic-co-glycolic) acid nanoparticles were manufactured as lipid ......
Bio Diagnostics, Analysis of bacteria-derived outer membrane vesicles using tunable resistive pulse sensing
TRPS Technology, Vesicles, Viruses & Bacteria, qNano, TRPSAccurate characterization of submicron particles within biological fluids presents a major challenge for a wide range of biomedical research. Detection, characterization and classification are difficult due to the presence of ......
High Resolution Particle Characterization to Expedite Development and Regulatory Acceptance of Nanomedicines
Drug Delivery & Vaccines, qNano, TRPSThe pharmaceutical industry as well as European and US governing agencies have indicated the need for more accurate, high resolution, characterization of complex drug materials, nanomedicines, to facilitate their development ......
Co-ordinated detection of microparticles using tunable resistive pulse sensing and fluorescence spectroscopy
TRPS Technology, qNano, TRPSTunable resistive pulse sensing (TRPS) has emerged as a useful tool for particle-by-particle detection and analysis of microparticles and nanoparticles as they pass through a pore in a thin stretchable ......
Optimized exosome isolation protocol for cell culture supernatant and human plasma
Vesicles, qEV, qNano, SEC, TRPSExtracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis of disease. However, there is currently limited information elucidating the most efficient methods for obtaining high ......
Improved Characterization of EV Preparations Based on Protein to Lipid Ratio and Lipid Properties
Vesicles, qNano, TRPSIn recent years the study of extracellular vesicles has gathered much scientific and clinical interest. As the field is expanding, it is becoming clear that better methods for characterization and ......
Differential detergent sensitivity of extracellular vesicle subpopulations
Vesicles, qNano, TRPSExtracellular vesicles (including exosomes, microvesicles and apoptotic bodies) are currently attracting rapidly increasing attention from various fields of biology due to their ability to carry complex information and act as ......
Possibilities and limitations of current technologies for quantification of biological extracellular vesicles and synthetic mimics.
Vesicles, qNano, TRPSNano-sized extracelullar vesicles (EVs) released by various cell types play important roles in a plethora of (patho)physiological processes and are increasingly recognized as biomarkers for disease. In addition, engineered EV ......
Observations of tunable resistive pulse sensing for exosome analysis: Improving system sensitivity and stability
Vesicles, qNano, TRPSSize distribution and concentration measurements of exosomes are essential when investigating their cellular function and uptake. Recently, a particle size distribution and concentration measurement platform known as Tunable Resistive...
Non-targeted effects of radiation exposure:recent advances and implications
Cells, Vesicles, qNano, TRPSThe target theory of radiation-induced effects has been challenged by numerous studies, which indicate that in addition to biological effects resulting from direct DNA damage within the cell, a variety ......
Purification and Characterization of extracellular vesicles from a 2 hyperthermophilic archaeon Thermococcus onnurineus NA1T
Vesicles, qNano, TRPSExtracellular vesicles (EVs) produced by a sulfur-reducing, hyperthermophilic archaeon, Thermococcus onnurineus NA1T (To), were purified and characterized. A maximum of four EV bands, showing buoyant densities between 1.1899 and 1.2828 ......
Mesenchymal-stem-cell-derived exosomes accelerate skeletal muscle regeneration
Vesicles, qNano, TRPSMesenchymal stem cell (MSC) transplantation is used for treatment of many diseases. The paracrine role of MSCs in tissue regeneration is attracting particular attention. We investigate the role of MSC ......
Glioblastoma-derived extracellular vesicles modify the phenotype of monocytic cells
Bio Diagnostics, TRPS Technology, Vesicles, qNano, TRPSGlioblastoma multiforme (GBM) is the most common primary brain tumor and is without exception lethal. GBMs modify the immune system, which contributes to the aggressive nature of the disease. Particularly, ......
The neutral sphingomyelinase pathway regulates packaging of the prion protein into exosomes
Vesicles, qEVPrion diseases are a group of transmissible, fatal neurodegenerative disorders associated with the misfolding of the host-encoded prion protein, PrPC , into a disease associated form, PrPSc. The transmissible prion ......
The non-targeted effects of radiation are perpetuated by exosomes
Bio Diagnostics, Cells, TRPS Technology, Vesicles, qNano, TRPSExosomes contain cargo material from endosomes, cytosol, plasma membrane and microRNA molecules, they are released by a number of non-cancer and cancer cells into both the extracellular microenvironment and body ......
Effect of exosomes derived from multipluripotent mesenchymal stromal cells on functional recovery and neurovascular plasticity in rats after traumatic brain injury
Drug Delivery & Vaccines, Vesicles, qNanoOBJECT Transplanted multipotent mesenchymal stromal cells (MSCs) improve functional recovery in rats after traumatic brain injury (TBI). In this study the authors tested a novel hypothesis that systemic administration of ......
Analysis of exosome purification methods using a model liposome system and tunable-resistive pulse sensing
Vesicles, qNanoExosomes are vesicles which have garnered interest due to their diagnostic and therapeutic potential. Isolation of pure yields of exosomes from complex biological fluids whilst preserving their physical characteristics is ......
Emergence of tunable resistive pulse sensing as a biosensor
Bio Diagnostics, Cells, Drug Delivery & Vaccines, TRPS Technology, Vesicles, Viruses & Bacteria, qNano, TRPSAbstract The article is written as a guide and tutorial that focuses on the use of Tunable Resistive Pulse Sensing, TRPS, as a platform for the detection of biological analytes. ......
Applications of Tunable Resistive Pulse Sensing
Bio Diagnostics, Drug Delivery & Vaccines, Micro & Nano Bubbles, TRPS Technology, Vesicles, Viruses & Bacteria, qNano, TRPSTunable resistive pulse sensing (TRPS) is an experimental technique that has been used to study and characterise colloidal particles ranging from approximately 50 nm in diameter up to the size ......
Reproducible extracellular vesicle size and concentration determination with tunable resistive pulse sensing
TRPS Technology, Vesicles, qNano, TRPSIntroduction: The size of extracellular vesicles (EVs) can be determined with a tunable resistive pulse sensor (TRPS). Because the sensing pore diameter varies from pore to pore, the minimum detectable ......
Chitinases Are Negative Regulators of Francisella novicida Biofilms
TRPS Technology, Viruses & Bacteria, qNano, TRPSBiofilms, multicellular communities of bacteria, may be an environmental survival and transmission mechanism of Francisella tularensis. Chitinases of F. tularensis ssp. novicida (Fn) have been suggested to regulate biofilm formation ......
Recent advances in the characterization of membrane morphology
TRPS Technology, qNano, TRPSThe characterization of membranes is important for membrane selection, membrane process diagnosis, and new membrane material design. Recent advances in membrane characterization techniques facilitate new membrane material design and...
Actuation of tunable elastomeric pores: Resistance measurements and finite element modelling
TRPS Technology, qNano, TRPSWe present a quantitative study of elastomeric nano- / micropore actuation using experiments and a finite element model. Ionic resistances have been measured for five specimens of two distinct sizes ......
Modelling of Resistive Pulse Sensing: Flexible Methods For Submicron Particles
Nanoparticles, TRPS Technology, qNano, TRPSNanopore science, the study of individual nanoscale pores within thin membranes, is a fast-growing field which presents numerous interesting problems for physicists and applied mathematicians. Nanopores are most commonly applied ......
High Resolution Characterization of Engineered Nanomaterial Dispersions in Complex Media Using Tunable Resistive Pulse Sensing Technology
Nanoparticles, TRPS Technology, qNano, TRPSIn vitro toxicity assessment of engineered nanomaterials (ENM), the most common testing platform for ENM, requires prior ENM dispersion, stabilization, and characterization in cell culture media. Dispersion inefficiencies and active ......
Calibration-less sizing and quantitation of polymeric nanoparticles and viruses with quartz nanopipets.
Nanoparticles, qNano, TRPSThe feasibility of using quartz nanopipets as simple and cost-effective Coulter counters for calibration-less quantitation and sizing of nanoparticles by resistive pulsing sensing (RPS) was investigated. A refined theory was ......
Chitinases Are Negative Regulators of Francisella novicida Biofilms
TRPS Technology, Viruses & Bacteria, qNano, TRPSBiofilms, multicellular communities of bacteria, may be an environmental survival and transmission mechanism of Francisella tularensis. Chitinases of F. tularensis ssp. novicida (Fn) have been suggested to regulate biofilm formation ......
Monitoring bacterial growth using tunable resistive pulse sensing with a pore-based technique
Cells, TRPS Technology, Viruses & Bacteria, qNano, TRPSA novel bacterial growth monitoring method using a tunable resistive pulse sensor (TRPS) system is introduced in this study for accurate and sensitive measurement of cell size and cell concentration ......
Measuring mass of nanoparticles and viruses in liquids with nanometer-scale pores
Nanoparticles, Viruses & Bacteria, qNano, TRPSNanopores have recently been developed for the detection and physical characterization of nanoparticles, viruses, proteins, nucleic acids, and other macromolecules in liquids. The method provides the ability to rapidly estimate ......
Quantitative real-time single particle analysis of virions
TRPS Technology, Viruses & Bacteria, qViro, TRPSProviding information about single virus particles has for a long time been mainly the domain of electron microscopy. More recently, technologies have been developed—or adapted from other fields, such as ......
Physical and acoustical properties of fluorocarbon nanoparticles
Bio Diagnostics, qNano, TRPSIn this thesis, acoustical and other physical properties of soft submicron suspensions were investigated in order to provide invaluable clues for their adaptation in theragnostic applications. Two types of dispersions ......
Small molecule detection in solution via the size contraction response of aptamer functionalized nanoparticles
Bio Diagnostics, Nanoparticles, TRPS Technology, qNano, TRPSWe demonstrate a simple new sensor design that exploits aptamer functionalized nanoparticles (NPs) to transduce the signal of aptamer receptors binding to target small molecules. An aptamer capable of binding ......
Assessment of PEG on polymeric particles surface, a key step in drug carrier translation
Drug Delivery & Vaccines, Nanoparticles, TRPS Technology, qNano, TRPSInjectable drug nanocarriers have greatly benefited in their clinical development from the addition of a superficial hydrophilic corona to improve their cargo pharmacokinetics. The most studied and used polymer for ......
Nanobiotechnology For Medical Diagnostics
TRPS Technology, Viruses & Bacteria, qNano, TRPSTraditional core areas of chemical engineering education are being extended by new expertise in science and engineering at the molecular and nanometer scale. Chemical engineers have been pursuing a dynamic ......
Specific targeting of tumor cells by lyophilisomes functionalized with antibodies
Drug Delivery & Vaccines, Nanoparticles, qNano, TRPSLyophilisomes are a novel class of proteinaceous biodegradable nano/micro drug delivery capsules prepared by freezing, annealing and Iyophilization. In the present study, lyophilisomes were functionalized for active targeting by antibody ......
Quantification and size-profiling of extracellular vesicles using tunable resistive pulse sensing.
Vesicles, qNano, TRPSExtracellular vesicles (EVs), including ‘microvesicles’ and ‘exosomes’, are highly abundant in bodily fluids. Recent years have witnessed a tremendous increase in interest in EVs. EVs have been shown to play ......
Characterization and deep sequencing analysis of exosomal and non-exosomal miRNA in human urine
Bio Diagnostics, Vesicles, qNano, TRPSMicro RNAs (miRNAs) have been shown to circulate in biological fluids and are enclosed in vesicles such as exosomes; they are present in urine and represent a noninvasive methodology to ......
Toward routine detection of extracellular vesicles in clinical samples
Bio Diagnostics, Vesicles, qNano, TRPSThe majority, if not all, of human cell types secrete extracellular vesicles (EVs) into their environment, at least partly as a means of intercellular communication. These secreted vesicles can be ......
Bioinspired protein microparticles fabrication by peptide mediated disulfide interchange
Drug Delivery & Vaccines, Micro & Nano Bubbles, Nanoparticles, Vesicles, qNano, TRPSIn this article, we report an innovative green chemistry approach for the fabrication of protein microparticles based on peptide mediated disulfide interchange reactions. The concept is based on using a ......
Exosomes provide a protective and enriched source of miRNA for biomarker profiling compared to intracellular and cell-free blood
Vesicles, qNano, TRPSIntroduction microRNA (miRNA) are small non-coding RNA species that are transcriptionally processed in the host cell and released extracellularly into the bloodstream. Normally involved in post-transcriptional gene silencing, the...
Critical role of extracellular vesicles in modulating the cellular effects of cytokines
Bio Diagnostics, Vesicles, qNano, TRPSUnder physiological and pathological conditions, extracellular vesicles (EVs) are present in the extracellular compartment simultaneously with soluble mediators. We hypothesized that cytokine effects may be modulated by EVs, the recently ......
Exosome-formed synthetic microRNA-143 is transferred to osteosarcoma cells and inhibits their migration
Bio Diagnostics, Drug Delivery & Vaccines, Vesicles, qNano, TRPSIn this article, we report an innovative green chemistry approach for the fabrication of protein microparticles based on peptide mediated disulfide interchange reactions. The concept is based on using a ......
Protocol Standardization Reveals MV Correlation to Healthy Donor BMI
Bio Diagnostics, Vesicles, qNano, TRPSMicrovesicles (MVs) are cell-derived vesicles which are of interest in a clinical setting, as they may be predictive of early signs of disease and/or of treatment progression. However, there are ......
Platelet microparticles: detection and assessment of their paradoxical functional roles in disease and regenerative medicine
Bio Diagnostics, Cells, Vesicles, qNano, TRPSThere is increasing research on and clinical interest in the physiological role played by platelet microparticles (PMPs). PMPs are 0.1-1-µm fragments shed from plasma membranes of platelets that are undergoing ......
Lipoprotein-apheresis reduces circulating microparticles in individuals with familial hypercholesterolemia
Vesicles, qNano, TRPSLipoprotein-apheresis (apheresis) removes LDL-cholesterol in patients with severe dyslipidemia. However, reduction is transient, indicating that the long-term cardiovascular benefits of apheresis may not solely be due to LDL removal....
Particle size distribution of exosomes and microvesicles by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing
Bio Diagnostics, Vesicles, qNano, TRPSBackground Enumeration of extracellular vesicles has clinical potential as a biomarker for disease. In biological samples, the smallest and largest vesicles typically differ 25-fold in size, 300,000-fold in concentration, 20,000-fold ......
Single-step isolation of extracellular vesicles by size-exclusion chromatography
Vesicles, qNano, SEC, TRPSBackground: Isolation of extracellular vesicles from plasma is a challenge due to the presence of proteins and lipoproteins. Isolation of vesicles using differential centrifugation or density-gradient ultracentrifugation results in...
Detecting Exosomes Specifically: A Multiplexed Device Based on Alternating Current Electrohydrodynamic Induced Nanoshearing
Bio Diagnostics, Vesicles, qNanoExosomes show promise as noninvasive biomarkers for cancer, but their effective capture and specific detection is a significant challenge. Herein, we report a multiplexed microfluidic device for highly specific capture ......
Small interfering ribonucleic acid delivery with phase-shift nanoemulsions
Drug Delivery & Vaccines, qNano, TRPSABSTRACT Acoustic cavitation offers a unique approach to small interfering RNA (siRNA) delivery compared to current methods. Typically, preformed microbubbles are used as cavitation nuclei to permeabilize cells and facilitate ......
Microscopy and tunable resistive pulse sensing characterization of the swelling of pH-responsive, polymeric expansile nanoparticles
Drug Delivery & Vaccines, Nanoparticles, TRPS Technology, qNano, TRPSPolymeric expansile nanoparticles (eNPs) that respond to a mildly acidic environment by swelling with water and expanding 2–10× in diameter represent a new responsive drug delivery system. Here, we use ......
Small interfering ribonucleic acid delivery with phase-shift nanoemulsions
Drug Delivery & Vaccines, qNano, TRPSABSTRACT Acoustic cavitation offers a unique approach to small interfering RNA (siRNA) delivery compared to current methods. Typically, preformed microbubbles are used as cavitation nuclei to permeabilize cells and facilitate ......
Amphiphilic Colloidal Surfactants Based on Electrohydrodynamic Co-jetting
Nanoparticles, qNano, TRPSA novel synthetic route for the preparation of amphiphilic Janus particles based on electrohydrodynamic cojetting has been developed. In this approach, selective encapsulation of hydrophobic fluorodecyl-polyhedral oligomeric...
Fast piezoelectric actuation of an elastomeric micropore
TRPS Technology, qNano, TRPSA viscoelastic membrane unit fabricated from thermoplastic polyurethane, with a formed single micropore, was interfaced to a piezoelectric actuator to demonstrate rapid actuation of the pore. Changes in through-pore conductance ......
Reconfigurable Shapes and Assemblies of Anisotropic Particles
Nanoparticles, qNano, TRPSThis dissertation describes dynamically reconfigurable particles that can undergo permanent or reversible changes in sizes and shapes depending on the surrounding environment. Electrohydrodynamic co-jetting provides the key for the...
Size and charge characterisation of a submicrometre oil-in-water emulsion using resistive pulse sensing with tunable pores
Nanoparticles, TRPS Technology, qNano, TRPSResistive pulse sensing (RPS) with tunable pores (TPs) has been used to investigate an oil-in-water emulsion stabilised with β-lactoglobulin (BLG). The mode of the droplet size distribution steadily increased over ......
Colloidal properties of nanoparticular biogenic selenium govern environmental fate and bioremediation effectiveness
Nanoparticles, TRPS Technology, qNano, TRPSMicrobial selenium (Se) bioremediation is based on conversion of water soluble, toxic Se oxyanions to water insoluble, elemental Se. Formed biogenic elemental Se is of nanometer size, hampering straightforward separation ......
The characterisation and measurement of biomedically relevant nanomaterials
Drug Delivery & Vaccines, TRPS Technology, qNano, TRPSNanomaterial metrology is fundamental to understanding the physical properties of nanomaterials and their environmental interactions. Particles, in the nano and sub-micrometer regimes, are attractive materials for use in biomedical...
Role of antifeeding prophage (Afp) protein Afp16 in terminating the length of the Afp tailocin and stabilizing its sheath
TRPS Technology, Viruses & Bacteria, qNano, TRPSThe Serratia entomophila antifeeding prophage Afp, forms a phage-tail-like particle that acts on the New Zealand grass grub, Costelytra zealandica with a 3-day LD50 of approximately 500 Afp particles per ......
A Gel Filtration-Based Method for the Purification of Infectious Rotavirus Particles for Environmental Research Applications
Viruses & Bacteria, qNano, TRPSThis article describes a rapid method for purifying infectious rotavirus particles from cell culture for environmental research. The method is based on size-exclusion chromatography using TOSOH TSKgel® G5000PWXL-CP with a ......
Detection of Panton-Valentine Leukocidin DNA from methicillin-resistant Staphylococcus aureus by resistive pulse sensing and loop-mediated isothermal amplification with gold nanoparticles
Bio Diagnostics, Nanoparticles, TRPS Technology, qNano, TRPSThis report describes a novel diagnostic assay for rapid detection of the Panton-Valentine Leukocidin (PVL) toxin of methicillin-resistant Staphylococcus aureus (MRSA) utilizing resistive pulse sensing (RPS), loop-mediated isothermal...
Tunable resistive pulse sensing as a tool to monitor analyte induced particle aggregation
Bio Diagnostics, TRPS Technology, qNano, TRPSAnalytical technologies based upon superparamagnetic beads, SPBs, offer a rapid, simple and inexpensive way of separating and purifying the target analyte prior to detection. The SPBs can perform the capture, ......
Microscopy and tunable resistive pulse sensing characterization of the swelling of pH-responsive, polymeric expansile nanoparticles
Drug Delivery & Vaccines, Nanoparticles, TRPS Technology, qNano, TRPSPolymeric expansile nanoparticles (eNPs) that respond to a mildly acidic environment by swelling with water and expanding 2–10× in diameter represent a new responsive drug delivery system. Here, we use ......
Detection of target-probe oligonucleotide hybridization using synthetic nanopore resistive pulse sensing
Bio Diagnostics, Nanoparticles, TRPS Technology, qNano, TRPSAbstract Despite the plethora of DNA sensor platforms available, a portable, sensitive, selective and economic sensor able to rival current fluorescence-based techniques would find use in many applications. In this ......
A comparative study of submicron particle sizing platforms: Accuracy, precision and resolution analysis of polydisperse particle size distributions
TRPS Technology, qNano, TRPSThe particle size distribution (PSD) of a polydisperse or multimodal system can often be difficult to obtain due to the inherent limitations in established measurement techniques. For this reason, the ......
ISEV position paper: extracellular vesicle RNA analysis and bioinformatics
Bio Diagnostics, Vesicles, qNano, TRPSExtracellular vesicles (EVs) are the collective term for the various vesicles that are released by cells into the extracellular space. Such vesicles include exosomes and microvesicles, which vary by their ......
Surface Glycosylation Profiles of Urine Extracellular Vesicles
Bio Diagnostics, Vesicles, qNano, TRPSUrinary extracellular vesicles (uEVs) are released by cells throughout the nephron and contain biomolecules from their cells of origin. Although uEV-associated proteins and RNA have been studied in detail, little ......
Label-free optical monitoring of surface adhesion of extracellular vesicles by grating coupled interferometry
Vesicles, qNano, TRPSIn this proof-of-principle study a label-free optical sensor is demonstrated to monitor the surface adhesion of extracellular vesicles secreted by live cells on to various extracellular matrix proteins....
Active caspase-3 is removed from cells by release of caspase-3-enriched vesicles
Cells, Vesicles, qNano, TRPSCleavage of Rho associated Coiled Coil kinase I (ROCK I) by caspase-3 contributes to membrane blebbing. Whether caspase-3 and ROCK I also play a role in the release of membrane ......
Endometrial exosomes/microvesicles in the uterine microenvironment: A new paradigm for embryo-endometrial cross talk at implantation
Bio Diagnostics, Vesicles, qNano, TRPSExosomes are nanoparticles (~100 nm diameter) released from cells, which can transfer small RNAs and mRNA via the extracellular environment to cells at distant sites. We hypothesised that exosomes or ......
Human adipose tissue-derived mesenchymal stem cells secrete functional neprilysin-bound exosomes
Bio Diagnostics, Vesicles, qNano, TRPSAlzheimer’s disease (AD) is characterized by the accumulation of β-amyloid peptide (Aβ) in the brain because of an imbalance between Aβ production and clearance. Neprilysin (NEP) is the most important ......
Standardization of sample collection,isolation and analysis methods inextracellular vesicle research
Bio Diagnostics, Vesicles, qNano, TRPSThe emergence of publications on extracellular RNA (exRNA) and extracellular vesicles (EV) has highlighted the potential of these molecules and vehicles as biomarkers of disease and therapeutic targets. These findings ......
Innovation in detection of microparticles and exosomes
Vesicles, qNano, TRPSCell-derived or extracellular vesicles, including microparticles and exosomes, are abundantly present in body fluids such as blood. Although such vesicles have gained strong clinical and scientific interest, their detection is ......
Polymer−Liposome Complexes with a Functional Hydrogen-Bond Cross-Linker for Preventing Protein Adsorption and Improving Tumor Accumulation
Drug Delivery & Vaccines, Vesicles, qNano, TRPSTo solve biostability and organ distribution problems in polymer-coated liposomes, this study developed tumor–extracellular matrix pH-induced targeting polymer–liposome complexes (ECM-targeting liposomes) that are highly stable in a...
Quantification of nanosized extracellular membrane vesicles with scanning ion occlusion sensing
Vesicles, qNano, TRPSBACKGROUND: Cells secrete different types of membrane vesicles (MVs), which may act as important entities in normal human physiology and in various pathological processes. The established methods for quantification of ......
Resistive pulse sensing of magnetic beads and supraparticle structures using tunable pores.
Nanoparticles, TRPS Technology, qNano, TRPSTunable pores (TPs) have been used for resistive pulse sensing of 1 μm superparamagnetic beads, both dispersed and within a magnetic field. Upon application of this field, magnetic supraparticle structures ......
Closing the Nanotechnology Workforce Gap
Nanoparticles, qNano, TRPSThe National Science Foundation projects a global demand of 6 million nanotechnology workers by 2020; however, only 400,000 nanotech workers are currently in the global workforce, and most of them ......
Roles of Proteins, Polysaccharides, and Phenolics in Haze Formation in White Wine via Reconstitution Experiments
TRPS Technology, qNano, TRPSResidual proteins in finished wines can aggregate to form haze. To obtain insights into the mechanism of protein haze formation, a reconstitution approach was used to study the heat-induced aggregation ......
Resistive pulse sensing of magnetic beads and supraparticle structures using tunable pores.
Nanoparticles, TRPS Technology, qNano, TRPSTunable pores (TPs) have been used for resistive pulse sensing of 1 μm superparamagnetic beads, both dispersed and within a magnetic field. Upon application of this field, magnetic supraparticle structures ......
Tuning Particle Velocity and Measurement Sensitivity by Changing Pore Sensor Dimensions
TRPS Technology, qNano, TRPSTuning the size of an elastic pore sensor enables the control of both the measurement sensitivity and particle velocity through the pore. Increasing the pore size by stretching the pore ......
A variable pressure method for characterizing nanoparticle surface charge using pore sensors
TRPS Technology, qNano, TRPSA novel method using resistive pulse sensors for electrokinetic surface charge measurements of nanoparticles is presented. This method involves recording the particle blockade rate while the pressure applied across a ......
Modeling Elastic Pore Sensors for Quantitative Single Particle Sizing
Nanoparticles, TRPS Technology, qNano, TRPSAn empirically derived model of how the dimensions of a size-tunable elastic pore sensor change with applied membrane stretch is presented. Quantitative modeling of the conical pore dimensions, in conjugation ......
Simultaneous Size and Zeta-Potential Measurements of Individual Nanoparticles in Dispersion Using Size-Tunable Pore Sensors
Nanoparticles, TRPS Technology, qNano, TRPSThe prospect of characterizing individual nanoparticles, molecules, or DNA base pairs has generated considerable interest in resistive pulse sensing. In addition to size and concentration analysis, this technique also has ......
Emerging techniques for submicrometer particle sizing applied to Stober silica
Nanoparticles, TRPS Technology, qNano, TRPSThe accurate characterization of submicrometer and nanometer sized particles presents a major challenge in the diverse applications envisaged for them including cosmetics, biosensors, renewable energy, and electronics. Size is one ......
Emerging techniques for submicrometer particle sizing applied to Stober silica
Nanoparticles, TRPS Technology, qNano, TRPSThe accurate characterization of submicrometer and nanometer sized particles presents a major challenge in the diverse applications envisaged for them including cosmetics, biosensors, renewable energy, and electronics. Size is one ......
Biotin-and Glycoprotein-Coated Microspheres: Potential Surrogates for Studying Filtration of Cryptosporidium parvum in Porous Media
Nanoparticles, Viruses & Bacteria, qNano, TRPSCryptosporidium parvum is a waterborne pathogen, yet no suitable surrogate has been established for quantifying its filtration removal in porous media. Carboxyl polystyrene microspheres with size, density, and shape similar ......
Magnetised thermo responsive lipid vehicles for targeted and controlled lung drug delivery
Drug Delivery & Vaccines, Nanoparticles, qNano, TRPSPurpose Conditions such as lung cancer currently lack non-invasively targetable and controlled release topical inhalational therapies. Superparamagnetic iron-oxide nanoparticles (SPIONs) have shown promising results as a targetable...
Tunable pores for measuring concentrations of synthetic and biological nanoparticle dispersions
Drug Delivery & Vaccines, TRPS Technology, Viruses & Bacteria, qNano, TRPSAbstract Scanning ion occlusion sensing (SIOS), a technique that uses a tunable pore to detect the passage of individual nano-scale objects, is applied here for the rapid, accurate and direct ......
Rapid and Label-Free Single-Nucleotide Discrimination via an Integrative Nanoparticle-Nanopore Approach
Bio Diagnostics, qNano, TRPSSingle-nucleotide polymorphism (SNP) is an important biomarker for disease diagnosis, treatment monitoring, and development of personalized medicine. Recent works focused primarily on ultrasensitive detection, while the need for rapid...
Resistive Pulse Sensing of Analyte-Induced Multicomponent Rod Aggregation Using Tunable Pores
Bio Diagnostics, TRPS Technology, qNano, TRPSResistive pulse sensing is used to monitor individual and aggregated rod-shaped nanoparticles as they move through tunable pores in elastomeric membranes. By comparing particles of similar dimensions, it is demonstrated ......
Lyophilisomes as a new generation of drug delivery capsules
Drug Delivery & Vaccines, Nanoparticles, TRPS Technology, qNano, TRPSNanoparticulate drug delivery systems are currently explored to overcome critical challenges associated with classical administration forms. In this study, we present a drug delivery system based on a novel class ......
Exploring the fate of liposomes in the intestine by dynamic in vitro lipolysis
Drug Delivery & Vaccines, Nanoparticles, TRPS Technology, qNano, TRPSLiposomes are generally well tolerated drug delivery systems with a potential use for the oral route. However, little is known about the fate of liposomes during exposure to the conditions ......
Characterization of a Nanoparticulate Drug Delivery System Using Scanning Ion Occlusion Sensing
Drug Delivery & Vaccines, Nanoparticles, TRPS Technology, qNano, TRPSPurpose To explore the application of scanning ion occlusion sensing (SIOS) as a novel technology for characterization of nanoparticles. Methods Liposomes were employed as model nanoparticles. The size distribution of ......
Alternative methods for characterization of extracellular vesicles
Bio Diagnostics, Vesicles, qNano, TRPSExtracellular vesicles (ECVs) are nano-sized vesicles released by all cells in vitro as well as in vivo. Their role has been implicated mainly in cell-cell communication, but also in disease ......
Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters
Vesicles, qNano, TRPSNumerous diseases, recently reported to associate with elevated microvesicle/microparticle (MP) counts, have also long been known to be characterized by accelerated immune complex (IC) formation. The goal of this study ......
Tunable Elastomeric Nanopores
Nanoparticles, TRPS Technology, Viruses & Bacteria, TRPSAbstract A general introduction to tunable elastomeric nanopore technology is presented. Tunable nanopores have been used to detect particles ranging from dsDNA to 10 μm diameter spheres using the resistive ......
Resistive pulse asymmetry for nanospheres passing through tunable submicron pores
TRPS Technology, TRPSAsymmetric resistive pulses caused by nanoparticles passing through tunable nanopores have been recorded and studied using a semianalytic physical model. Experiments used 220 nm diameter carboxylate-modified polystyrene spheres,...
Advances in resistive pulse sensors: devices bridging the void between molecular and microscopic detection
TRPS Technology, TRPSSince the first reported use of a biological ion channel to detect differences in single stranded genomic base pairs in 1996, a renaissance in nanoscale resistive pulse sensors has ensued. ......
Investigative study of nucleic acid-gold nanoparticle interactions using laser-based techniques, electron microscopy, and resistive pulse sensing with a nanopore
Nanoparticles, TRPS Technology, TRPSIn this study, we employ a range of analytical tools to study the interactions between a mixed base peptide nucleic acid (PNA, 22-mer) probe and gold nanoparticles (AuNP). The binding ......
Tunable Elastomeric Nanopores
Nanoparticles, TRPS Technology, Viruses & Bacteria, TRPSAbstract A general introduction to tunable elastomeric nanopore technology is presented. Tunable nanopores have been used to detect particles ranging from dsDNA to 10 μm diameter spheres using the resistive ......
Quantitative Sizing of Nano/Microparticles with a Tunable Elastomeric Pore Sensor
Nanoparticles, TRPS Technology, Viruses & Bacteria, TRPSThe use of a “size-tunable” polyurethane resistive pulse sensor for quantitative sizing of nano- and microparticles is presented. A linear relationship, as first suggested by Maxwell, between particle volume and ......
Use of tunable nanopore blockade rates to investigate colloidal dispersions
TRPS Technology, qNano, TRPSTunable nanopores fabricated in elastomeric membranes have been used to study the dependence of ionic current blockade rate on the concentration and electrophoretic mobility of particles in aqueous suspensions. A ......
Pressure dependence of particle transport through resizable nanopores
Nanoparticles, TRPSAbstract: Nanoparticles between 200 nm and 780 nm in diameter have been electronically detected as they passed through resizable nanopores. The dominant mechanism of particle transport was via pressure-driven flow. ......
Tunable nano/micropores for particle detection and discrimination: scanning ion occlusion spectroscopy
TRPS Technology, TRPSConventional pore technologies are limited in the size range of structures they can analyze by the fixed size of the pore. A novel stretchable pore which can be tuned to ......
Portable nanoparticle quantization using a resizable nanopore instrument-The IZON qNano
Nanoparticles, Vesicles, Viruses & Bacteria, qNano, TRPSThis paper demonstrates initial results with a novel instrument for nanoparticle detection and quantization, called the “qNano.” The qNano instrument provides a label-free method for detection of charged particles passing ......
Reversible mechanical actuation of elastomeric nanopores
TRPS Technology, TRPSAbstract Mechanical resizing of individual nanopores in a thermoplastic polyurethane elastomer has been characterized. Specimen nanopores were conical, with smaller hole dimensions of the order of tens to hundreds of ......
Dynamically resizable nanometre-scale apertures for molecular sensing
TRPS Technology, TRPSAbstract An easily fabricated resizable nanometre-scale aperture provides a novel class of electrical device that can be deployed as a tunable molecular sensor. Here, we demonstrate that the metered penetration ......
