EV Concentration and RNA Extraction Kits to Supplement Your qEV Isolation Workflow

RNA Extraction From Extracellular Vesicles

The qEV RNA Extraction Kit enables repeatable and standardised extraction of high-quality RNA from samples enriched with extracellular vesicles (EVs).

Extracellular vesicle RNA (EV-RNA) is among the cargo carried and protected by EVs, alongside protein and other small molecules. As EV-RNA consists of both small RNAs (e.g., miRNAs) and larger RNAs (e.g., mRNAs), EV researchers require an extraction method capable of extracting RNA of all sizes and types.

Both EV isolation and RNA extraction methods impact upon the yield and purity of extracted EV-RNA. The qEV RNA Extraction Kit, paired with qEV columns, allows you to seamlessly standardise your EV-RNA workflow from starting sample to high yield, pure RNA. Crucially, the qEV RNA Extraction Kit isolates EV RNA without bias based upon either GC content or size, meaning that your RNA isolate will be truly representative.

Harness Magnetic Particles to Quickly Concentrate EVs

A clean and efficient system for concentrating EVs that have been isolated using qEV columns. The qEV Magnetic Concentration Kit contains Magnetic Nanotrap^® Extracellular Vesicle Particles produced for us by Ceres Nanosciences. Simply add the specified volume of Nanotrap^® Particles to your EV isolate, and incubate with rotation at room temperature for 10 minutes. After applying a strong magnet for 2 minutes, you can remove the supernatant to reveal your EV-containing pellet, ready for resuspension in a desired volume for your downstream application. A quick and clean EV concentration method which removes the need for centrifugation or time-consuming protocols.  

A simple solution for concentrating EV-containing samples

The qEV Concentration Kit is a particle-based concentration system for enriching purified extracellular vesicles (EVs), without the need for ultra-speed centrifugation or addition of reagents that alter the purity of EVs.

The qEV Concentration Kit captures intact EVs isolated using any of the qEV Columns producing elution volumes ranging from 600 μL to 20 mL. Using the qEV Concentration Kit, EVs can be concentrated to a customisable pellet, which can be used as it is for maximal EV enrichment, or resuspended in a desired volume and buffer type. Concentrated EVs are particularly suited to protein and RNA analysis. EVs concentrated using this kit are not suitable for functional assays.