1. You can optimise EV yield and minimise lipoprotein contamination by combining qEV columns, the AFC and ultrafiltration
María Fernández‐Rhodes et al. Defining the influence of size‐exclusion chromatography fraction window and ultrafiltration column choice on extracellular vesicle recovery in a skeletal muscle model. Journal of Extracellular Biology 2, (2023). https://doi.org/10.1002/jex2.85
2. Isolation methods compared; survey results show scalability of SEC largely underestimated in 2020/2021
Williams, S. et al. Comparison of extracellular vesicle isolation processes for therapeutic applications. Journal of Tissue Engineering 14 (2023). https://doi.org/10.1177/20417314231174609
3. Studying EV interstitial transport: effective removal of dye using qEV isolation
Sariano, P. A. et al. Convection and extracellular matrix binding control interstitial transport of extracellular vesicles. Journal of Extracellular Vesicles 12, (2023). https://doi.org/10.1002/jev2.12323
4. Clinical outcomes of sepsis are associated with patterns of EV caspase-1 activity and EV-miRNA markers
Li, P. et al. Circulating extracellular vesicles are associated with the clinical outcomes of sepsis. Frontiers in Immunology 14, (2023). https://doi.org/10.3389/fimmu.2023.1150564
5. Liver-derived EVs in fatty liver enhance progression of colorectal cancer liver metastasis
Wang, Z. et al. Extracellular vesicles in fatty liver promote a metastatic tumor microenvironment. Cell Metabolism (2023) https://doi.org/10.1016/j.cmet.2023.04.013
6. Therapeutic opportunities uncovered in study of sepsis-associated bacterial EVs
Laakmann, K. et al. Bacterial extracellular vesicles repress the vascular protective factor RNase1 in human lung endothelial cells. Cell Communication and Signaling 21, 111–111 (2023). https://doi.org/10.1186/s12964-023-01131-2
7. EV reporter development: progress and challenges in studying X-linked dystonia-parkinsonism
Maalouf, K. E. et al. Tracking human neurologic disease status in mouse brain/plasma using reporter-tagged, EV-associated biomarkers. Molecular Therapy (2023) https://doi.org/10.1016/j.ymthe.2023.05.011
8. Exploring the recognition of EVs by the cell membrane receptor Siglec-6
Schmidt, E. N. et al. Siglec-6 mediates the uptake of extracellular vesicles through a noncanonical glycolipid binding pocket. Nature Communications 14, 2327 (2023). https://doi.org/10.1038/s41467-023-38030-6
9. Multiple cell types contribute to altered EV profiles in systemic juvenile idiopathic arthritis
Maller, J. et al. Extracellular Vesicles in Systemic Juvenile Idiopathic Arthritis. Journal of Leukocyte Biology qiad059 (2023) https://doi.org/10.1093/jleuko/qiad059
10. Changes in circulating EVs unravelled in patients with severe COVID-19
Forte, D. et al. Circulating extracellular particles from severe COVID-19 patients show altered profiling and innate lymphoid cell-modulating ability. Frontiers in Immunology 14, (2023). https://doi.org/10.3389/fimmu.2023.1085610
