- Robinson et al. showed that size-exclusion chromatography (SEC) using qEV1 35 nm columns is a fast, simple and effective technique for separating extracellular vesicles (EVs) from small (1 mL) volumes of plasma. Such volumes are obtainable from a standard blood test and are in the volume range routinely stored by biobanks, making them well-suited for isolation by qEV1 columns.
- Bracht et al. demonstrated that SEC columns with a 70 nm pore size, including the qEV Gen 2 / 70 nm columns, yield higher purity EVs than columns with smaller pore sizes. Conversely, smaller pore sizes, such as the qEV Gen 2 / 35 nm column, provide higher recovery of EVs. The authors conclude that 70 nm columns are the best fit for their specific application — EV miRNA cargo analysis. To find out which column will work best for your needs, explore our qEV series comparison tool.
- Grenhas et al. advocated for the importance of standardisation in EV isolation methods to unlock their full potential for biomedical research. This study found that SEC-based EV separation resulted in both higher yields and improved reproducibility across replicates than sucrose cushion ultracentrifugation.
- Swatler et al. developed a standardised protocol using qEVoriginal columns to isolate large quantities of EVs for use in downstream functional studies. They isolated tumour-derived EVs and cultured them with T cells to investigate their effect on T cell phenotype. Their detailed end-to-end workflow is published to help other labs wishing to carry out similar studies.
- Drees et al. emphasised the need to consider regulatory requirements to enable clinical implementation of EV assays. The authors used qEVoriginal 70 nm columns, coupled with the AFC for automation, to develop a quality-controlled EV-miRNA assay, as a step towards regulatory compliance. Their ultimate aim is to be able to monitor treatment response in classical Hodgkin lymphoma patients using an EV-based liquid biopsy.
