Zeta potential measurement with TRPS

True zeta potential distributions provide unbiased results
Obtain the required certainty in your particle research
Much more precise & reliable than DLS based measurement
Understand the subtle changes from different formulations
Simultaneous size & zeta potential measurement
Measure individual particles, on a particle-by-particle basis
Charge measurement in physiological-strength buffer
Measure and analyse particles in the medium that they will be used
Fine scale precision
Measure and analyse biochemical interactions and content in new ways, e.g. drug loading
Much more precise and reliable than DLS based zeta measurement
Phase analysis light scattering (PALS), an ensemble technique based on laser doppler velocimetry, is the best known technology for the determination of zeta potential of nanoparticle suspensions. Although readily available, ensemble techniques (e.g. PALS), as opposed to single particle measurement techniques, such as TRPS, can only measure and calculate the average particle mobility and hence detailed single particle information is lost, in particular when measuring polydisperse samples. TRPS is the only available technology that provides simultaneous in-suspension information about particle size and zeta potential on a particle-by-particle basis, guaranteeing accurate analysis of multi-modal and polydisperse samples (see figure). Whilst a mixed sample of bare and carboxylated polystyrene spheres with equivalent sizes (~400 nm) but different zeta potentials was perfectly resolved with TRPS (red trace), the two particle populations could not be distinguished with PALS (Figure 1a, red trace).
Charge measurement in physiological-strength buffer
Particle dispersions and formulations are stabilized by electrostatic repulsion, steric hindrance, or a combination of these two forces. Particles will eventually aggregate in the absence of sufficient stabilisation. Researchers use zeta potential as an indicator of electrostatic stabilisation of particles. Zeta potential is a modelled quantity derived by measuring electrophoretic mobility of particles in suspension. Electrophoretic mobility is critically dependent on particle and solution properties (ionic strength, ionic composition, and viscosity). Thus, it’s important to perform zeta analysis for nanoparticles or nano-formulations in physiological buffer(s) designed for biological applications.
High-resolution single particle zeta analysis will provide an advanced understanding of particle behaviour in different pH and salt conditions and aid in monitoring particle corona evolution over time for biokinetic studies. Moreover, determination of accurate charge on each particle could provide potential insights into nano-bio interactions in varying physiological buffers, critical for determining particle stability and uptake; impacting the overall delivered particle dosage.