Exosome-based therapeutics: purification using semi-continuous multi-column chromatography

Moleirinho, Mafalda G., Ricardo JS Silva, Manuel JT Carrondo, Paula M. Alves, and Cristina Peixoto. "Exosome-based therapeutics: Purification using semi-continuous multi-column chromatography." Separation and Purification Technology 224 (2019): 515-523.

Extracellular vesicles, more particularly exosomes, are nanostructures of great medical interest. Similarly to other complex biopharmaceuticals such as virus, the purification of these nanovesicles is still a considerable challenge. Here we describe a proof-of-concept purification strategy based on the well-established size exclusion chromatography technique, operated in a semi-continuous mode. A two-column system was designed and experimentally validated. Immunoblotting for protein exosome markers and electron microscopy indicated the presence of exosomes, with its characteristic cup-shaped morphology. The isolated nanovesicles have a mode size of 128 nm and a concentration and size distribution very similar between the collected products. Importantly, after comparison with batch chromatography, we were able to increase the yield up to 83%, specific productivities in 167% and a 2.7-fold reduction in buffer consumption with the semi-continuous operation. Finally, we demonstrate that the developed process can be potentially applied in the large-scale production of exosome-based therapeutics.

Graphical abstract
Exosomes are small nanovesicles released by the cells with a potential therapeutic application. A semi-continuous size exclusion chromatography process was developed to support the purification of these nanovesicles that can be used as drug delivery vehicles, diagnostic tools and vaccines.

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