Exosomal piRNA sequencing reveals differences between heart failure and healthy patients

Yang, J., F. T. Xue, Y. Y. Li, W. Liu, and S. Zhang. "Exosomal piRNA sequencing reveals differences between heart failure and healthy patients." European review for medical and pharmacological sciences 22, no. 22 (2018): 7952-7961.

OBJECTIVE: Heart failure is a leading cause of cardiovascular mortality in in- dustrialized countries. Increasing evidence has highlighted the relationship between noncoding regulatory RNAs, especially microRNAs, and heart failure. However, few studies address the role of piRNAs in heart failure. Therefore, we compared exosomal piRNAs between heart fail- ure patients and healthy volunteers to investi- gate the role of piRNAs in heart failure.
PATIENTS AND METHODS: First, exosomes were isolated from the serum of heart failure patients and healthy volunteers. After confirm- ing exosome identity by electron microscopy, nanoparticle analysis, and Western blotting, RNA was extracted from exosomes. The expres- sion of piRNAs was then compared by RNA se- quencing and bioinformatics analysis.
RESULTS: Serum exosomes from heart failure patients and healthy volunteers were success- fully isolated and identified. Serum exosome presence was increased in heart failure patients compared to healthy volunteers. RNA sequenc- ing and bioinformatics analysis revealed that 585 piRNAs were upregulated in heart failure pa- tients, and 4,623 piRNAs were downregulated. Among these piRNAs, has-piR-020009 and has- piR-006426 were the most downregulated.
CONCLUSIONS: Collectively, a dramatic dif- ference in the expression of piRNAs in se- rum exosomes of heart failure patients was ob- served. Altogether, these data suggest that piR- NAs are potential biomarkers of heart failure and that serum exosome isolation may provide a clinically relevant source of piRNAs for se- quencing analysis.

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