The VEGF pathway has emerged as an important target for cancer therapy by blocking the development of malignant neovasculature, thus to reduce oxygen availability to the tumor and decrease its …
Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis
Exosomes, the smallest subset of extracellular vesicles (EVs), have recently attracted much attention in the scientific community. Their involvement in intercellular communication and molecular reprogramming of different cell types created …
Mesenchymal Stem Cell-Derived Exosomes Reduce A1 Astrocytes via Downregulation of Phosphorylated NFκB P65 Subunit in Spinal Cord Injury
Background/Aims: Neurotoxic A1 astrocytes are induced by inflammation after spinal cord injury (SCI), and the inflammation-related Nuclear Factor Kappa B (NFκB) pathway may be related to A1-astrocyte activation. Mesenchymal stem …
Chromatographic Approaches for Purification and Analytical Characterization of Extracellular Vesicles: Recent Advancements
Extracellular vesicles (EVs) are nanoscale particles released by cells under both physiological and pathological conditions, that mediate intercellular communication by delivering biomolecules (nucleic acids, proteins, and lipids). In the last …
Generation of therapeutic cells using extracellular components of target organs
The invention relates to an ex vivo generated population of tissue-specific anti-inflammatory macrophages and methods of making and using such macrophages
EGFR detection in extracellular vesicles of breast cancer patients through immunosensor based on silica-chitosan nanoplatform
In the present work, we designed a microfluidic electrochemical immunosensor with enough sensibility and precision to quantify epithermal growth factor receptor (EGFR) in plasma extracellular vesicles (EVs) of plasma from …
Microvesicle and stem cell compositions for therapeutic applications
Provided herein are stem cell and exosome compositions having therapeutic utility to treat a variety of diseases and disorders, e.g., cardiovascular disease, Duchenne muscular dystrophy, and fibrotic disease.
A Comparison of Clinically Relevant Sources of Mesenchymal Stem Cell-Derived Exosomes: Bone Marrow and Amniotic Fluid
Background/Purpose Exosomes may constitute a more practical alternative to live cells in select stem cell-based therapies. We sought to compare exosomes from two mesenchymal stem cell (MSC) sources relevant to …
Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging
Extracellular vesicles (EVs) have prevalent roles in cancer biology and regenerative medicine. Conventional techniques for characterising EVs including electron microscopy (EM), nanoparticle tracking analysis (NTA) and tuneable resistive pulse sensing …
MSC-derived exosomes ameliorate erectile dysfunction by alleviation of corpus cavernosum smooth muscle apoptosis in a rat model of cavernous nerve injury
Background This study investigated the therapeutic effects of MSC-derived exosomes (MSC-Exos) on erectile function in a rat model of cavernous nerve injury (CNI). Methods MSCs were isolated from rat bone …
Extracellular vesicle microRNA cargo is correlated with HPV‐status in oropharyngeal carcinoma
Background The incidence of human papilloma virus positive (HPV+) oropharyngeal squamous cell carcinoma (OPSCC) has increased rapidly in recent decades. These tumours have a favourable outcome compared to HPV‐negative (HPV‐) …
A Magnetic-Based Microfluidic Device for On-Chip Isolation and Detection of Tumor-Derived Exosomes
Exosomes are membrane-enclosed phospholipid extracellular vesicles which can act as mediators of intercellular communication. Although the original features endow tumor-derived exosomes great potential as biomarkers, efficient isolation and detection methods …
Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization
Abstract Interest in extracellular vesicles and in particular microvesicles and exosomes, which are constitutively produced by cells, is on the rise for their huge potential as biomarkers in a high …
Highly sensitive detection of exosomes by SERS using gold nanostar@Raman reporter@nanoshell structures modified with a bivalent cholesterol-labeled DNA anchor
Exosomes, as important signal transmitters, play a key role in intercellular communication, especially in cancer metastasis. There is considerable evidence that exosomes can be used as an indicator of cancer. …
A Comparison of an Optimized Ultracentrifugation Method versus Size- Exclusion Chromatography for Isolation of Exosomes from Human Serum
Exosomes are nanosized vesicles that are abundant in biological fluids. In recent years, exosomes have attracted increasing attention as their cargo may provide promising biomarkers for the early diagnosis of …
Colorectal Cancer-derived Small Extracellular Vesicles Establish an Inflammatory Pre-metastatic Niche in Liver Metastasis
Liver metastases develop in more than half of the patients with colorectal cancer (CRC) and are associated with a poor prognosis. The factors influencing liver metastasis of CRC are poorly …
Screening of red blood cells for extracellular vesicle content as a product quality indicator
BACKGROUND The controversy around the quality and clinical impact of stored and differentially manufactured red cell concentrates (RCCs) from different donor groups is ongoing. Current studies are limited by the …
Ebola Virus VP40 Modulates Cell Cycle and Biogenesis of Extracellular Vesicles
Background Ebola virus (EBOV) mainly targets myeloid cells; however, extensive death of T cells is often observed in lethal infections. We have previously shown that EBOV VP40 in exosomes causes …
Dopamine-loaded blood exosomes targeted to brain for better treatment of Parkinson's disease
Abstract Parkinson’s disease (PD), one of the most common movement and neurodegenerative disorders, is challenging to treat, largely because the blood-brain barrier blocks passage of most drugs. Here we find …
Provocative decompression causes diffuse vascular injury in mice mediated by microparticles containing interleukin-1β
Inflammatory mediators are known to be elevated in association with decompression from elevated ambient pressure, but their role in tissue damage or overt decompression sickness is unclear. Circulating microparticles (MPs) …