Virus characterisation at the Jenner Institute

The Jenner Institute, a world leading non-profit organisation for vaccine development located in Oxford, UK, has been applying the Tunable Resistive Pulse Sensing (TRPS)  measurement approach to assess their viral sample preparations. The Institute is developing promising new vaccine candidates against major global infectious diseases and currently has new vaccines against malaria, tuberculosis and HIV in field trials in the developing world.

Dr. Ali Turner, Vector Core Facility Deputy Manager, and Jake Matthews, a post-graduate researcher at the Institute, are using TRPS to further their research into vaccine vectors. Key parameters of interest include accurate measurement of viral titre as well as assessment of levels of aggregation within the virus samples. An understanding of the level of aggregation is particularly important to ensure the quality of the vaccine produced from the virus. Work is focusing on two viruses, Adenovirus and Modified Vaccinia Ankara (MVA) virus, both used extensively as vectors or delivery vehicles in both vaccinology and gene therapy.

Researchers at the institute contributed to a paper on particle sizing analysis using TRPS demonstrating the resolution capability of the qViro instrument for virus samples, clearly distinguishing single adenovirus particles from two particle aggregates.