qEVoriginal SEC columns


Rapid & Reliable Exosome Purification


Izon’s reusable qEVoriginal Size Exclusion Columns enable the rapid isolation of extracellular vesicles (EVs) from cell culture supernatants and complex biological fluids. Each column efficiently removes background proteins, lipids, solutes and other contaminants to improve the sensitivity and accuracy of downstream assays (e.g., TRPS, protein profiling, RNA profiling, etc.) while maintaining the biological properties of the EVs. Vesicles are isolated in 15 minutes allowing EV samples to be collected and analysed immediately. Columns are available off-the-shelf and quality assured so your isolations are reproducible from column to column.



Rapid & Reliable

It takes around 15 minutes to get a pure sample of intact vesicles, significantly more pure than is possible with other methods.


Standardisable & Reproducible

Each column is standardised, quality assured and certified to ISO 13485 standard (Medical Devices) making it perfect for clinical testing.


Pure & Clean Isolated Samples

Our qEV single columns provide clean and pure samples without affecting the structure and function of the exosomes.

Fast isolation, gentle on your
sample.



qEVoriginal Columns are very gentle on your vesicles ensuring purified EVs retain their biological function. Isolation takes 15 minutes, removes more than 99% of contaminating proteins and is reproducible from column-to-column. In comparison, vesicle preparation using ultracentrifugation takes from 2 to 96 hours to complete and shows variable recovery. Since samples are isolated without extreme centrifugal force, the risk of forming protein or vesicle aggregates that can contaminate your EVs is low with qEVoriginal. Unlike density-gradient ultracentrifugation, vesicles are isolated using phosphate-buffered saline solution (PBS). By avoiding the use of highly viscous and hyperosmotic sucrose gradients, the biological function of your vesicles post isolation is assured.


qEVoriginal specifications



  • Reusable up to 5 times

  • 500 µL sample volume

  • Removes >99% of contaminating background proteins

  • Void volume of 3 ml


  • 10 minute preparation time

  • 5 minute separation time

  • Removes bulk LDL/HDL less than 70 nm

  • >70 nm separation size



qEVoriginal specifications


  • Reusable up to 5 times

  • 250-500 µL sample volume

  • Removes >99% of contaminating background proteins

  • Removes up to 95% of contaminating HDL

  • Void volume of 3.0ml ±0.25ml

  • 10 minute preparation time

  • 5 minute separation time

  • >70 nm separation size