qEV Exosome Isolation


Isolate your exosomes in just 15 minutes


Izon’s qEV provides the most rapid and effective exosome/EV isolation system available. qEV is based on Size Exclusion Chromatography (SEC), and takes around 15 minutes to get a pure sample of intact vesicles, significantly purer than is possible with concentration methods such as ultra-centrifugation (UC) or precipitation reagent kits. The resulting samples are consistent, standardisable and repeatable, which is required for both research and clinical testing. qEV is now the Gold Standard for exosome/EV isolation.



Rapid, Simple & Reliable

Using a qEV column takes around 15 minutes to get a pure sample of intact vesicles. Reliability has been proven in a large scale inter-laboratory trial.


Standardisable & Reproducible

Each column is stardardised, quality assured and certified to ISO 13485 standard (Medical Devices) making it perfect for clinical testing.



Pure, Clean Isolation Samples

qEV columns provide clean and pure samples without affecting the structure and function of the exosomes.





Fastest. Easiest. Purest.


qEV offers fast isolation of EVs, removing more than 99% of soluble proteins in just 15 minutes. This makes qEV-isolated EVs suitable for electron microscopy, proteomics, and RNA analysis.


  • ISO 13485 Certified

    Quality certified to ISO 13485: 2016 – Medical Devices

  • Pure

    Removes > 99% of soluble proteins

  • Versatile

    qEV can be used for vesicles, viruses & more




The new standard in extracellular vesicle isolation.


Prior to the advent of SEC columns, UC was regarded as the gold standard for EV concentration and isolation. UC is time consuming and cannot provide a consistent standardised sample. The very high forces in UC, up to 200,000g, affect, disrupt and aggregate the vesicles, which may in turn invalidate much of the research. Moreover, aggregated proteins and nucleic acid contaminants are present in the pellet containing the EVs. Density gradient centrifugation (DGC) aims to improve the purity of UC derived exosomes but increases the complexity and time of the procedure even further. Standardised sampling across different laboratories is not achievable with either UC or DGC.

Precipitation reagent kits were adapted for exosome/EV use from virus preparation. They are typically PEG based and supposedly sediment the exosomes only.  Published independent data indicates these fractions are heavily contaminated with non-EV material because these reagents cause co-precipitation of proteins, lipoproteins and other biological components. The leading researchers in the EV field no longer use or recommend these products. qEV SEC columns provide clean samples without affecting the structure or function of the EV. Precipitation reagent kits provide dirty samples that vary from batch to batch and are no longer recommended for exosome purification.



The new standard in extracellular vesicle isolation.


Prior to the advent of SEC columns, UC was regarded as the gold standard for EV concentration and isolation. UC is time consuming and cannot provide a consistent standardised sample. The very high forces in UC, up to 200,000g, affect and disrupt the vesicles, which may in turn invalidate much of the research. Moreover, aggregated proteins and nucleic acid contaminants are present in the pellet containing the EVs. Density gradient centrifugation (DGC) aims to improve the purity of UC derived exosomes but increases the complexity and time of the procedure even further. Standardised sampling across different laboratories is not achievable with either UC or DGC.

Precipitation reagent kits were adapted for exosome/EV use fromvirus preparation. They are typically PEG based and supposedly sediment the exosomes only.  Published independent data indicates these fractions are heavily contaminated with non-EV material because these reagents cause co-precipitation of proteins, lipoproteins and other biological components. The leading researchers in the EV field no longer use or recommend these products. qEV SEC columns provide clean samples without affecting the structure or function of the EV. Reagent kits provide dirty samples that vary from batch to batch and are no longer recommended for exosome purification.

The qEV range


qEVsingle

  • 100-150 µL

    Sample loading

  • Ideal for clinical samples, RT-PCR

    ISO 13485 certified. Optimised for small samples. No RNA carryover.

  • Single Use

    Small and low cost


qEVsingle

qEVoriginal

  • 500 µL

    Sample loading

  • Ideal for higher volume research use

    ISO 13485 Certified. The original, and most popular qEV column.

  • Reusable

    Can be cleaned and reused, making it very cost efficient.


qEVoriginal

qEV2

qEV2 SEC Column

  • 2 ml

    Sample loading

  • Ideal for larger clinical samples & RNA preparation

    ISO 13485 certified.

  • Reusable

    Very cost efficient for 2mL sample volumes


qEV2

qEV10

  • 10 ml

    Sample loading

  • Ideal for large volume cell culture supernatant

    ISO 13485 certified.

  • Reusable

    Very cost efficient for larger sample volumes


qEV10

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