qEV Exosome Isolation


Rapid, High Precision Exosome Isolation.


qEV provides the most rapid and effective EV/exsosome isolation system available. qEV is based on Size Exclusion Chromatography (SEC), and takes around 15 minutes to get a pure sample of intact exosomes, significantly purer than is possible with concentration methods such as ultra-centrifugation (UC) or precipitation reagent kits. The resulting samples are consistent, standardisable and repeatable, which is required for both research and clinical testing. qEV is now the gold standard for exosome isolation.



Rapid, Simple & Reliable

With qEV it takes around 15 minutes to get a pure sample of intact exosomes. Reliability has been proven in a large scale inter-laboratory trial.


Standardisable & Reproducible

Each column is stardardised, quality assured and certified to ISO 13485 standard (Medical Devices) making it perfect for clinical testing.



Pure, Clean Isolation Samples

qEV columns provide clean and pure samples without affecting the structure and function of the exosomes.





Rapid. Gentle. Precise.


qEV offers fast, gentle & precise isolation exosomes, removing more than 99% of soluble proteins. This makes qEV-isolated EVs/exosomes suitable for electron microscopy, proteomics, RNA analysis, and physical measurement.


  • ISO 13485 Certified

    Quality certified to ISO 13485: 2016 – Medical Devices

  • Pure

    Removes > 99% of soluble proteins

  • Optimised For Your Research

    The rapidly growing qEV range now includes 8 different columns.




The new standard in exosome isolation.


Prior to the advent of SEC columns, UC was regarded as the gold standard for EV concentration and isolation. UC is time consuming and cannot provide a consistent standardised sample. The very high forces in UC, up to 200,000g, affect, disrupt and aggregate the vesicles, which may in turn invalidate much of the research. Moreover, aggregated proteins and nucleic acid contaminants are present in the pellet containing the EVs. Density gradient centrifugation (DGC) aims to improve the purity of UC derived exosomes but increases the complexity and time of the procedure even further. Standardised sampling across different laboratories is not achievable with either UC or DGC.

Precipitation reagent kits have been adapted for exosome/EV purification. They are typically PEG based and sediment a wide range of product, not just EVs. Published independent data indicates these fractions are heavily contaminated with non-EV material because these reagents cause co-precipitation of proteins, lipoproteins and other biological components. qEV SEC columns provide clean samples without affecting the structure or function of the EV. Precipitation reagent kits provide dirty samples that vary from batch to batch. The leading researchers in the EV field no longer use or recommend precipitation products.





The new standard in extracellular vesicle isolation.


Prior to the advent of SEC columns, UC was regarded as the gold standard for EV/exosome concentration and isolation. UC is time consuming and cannot provide a consistent standardised sample. The very high forces in UC, up to 200,000g, affect and disrupt the vesicles, which may in turn invalidate much of the research. Moreover, aggregated proteins and nucleic acid contaminants are present in the pellet containing the EVs. Density gradient centrifugation (DGC) aims to improve the purity of UC derived exosomes but increases the complexity and time of the procedure even further. Standardised sampling across different laboratories is not achievable with either UC or DGC.

Precipitation reagent kits were adapted for exosome/EV use fromvirus preparation. They are typically PEG based and supposedly sediment the exosomes only.  Published independent data indicates these fractions are heavily contaminated with non-EV material because these reagents cause co-precipitation of proteins, lipoproteins and other biological components. The leading researchers in the EV field no longer use or recommend these products. qEV SEC columns provide clean samples without affecting the structure or function of the EV. Reagent kits provide dirty samples that vary from batch to batch and are no longer recommended for exosome purification.





How qEV Isolation Works


qEV is based on size exclusion chromatography (SEC). Size exclusion chromatography uses a stationary phase consisting of porous resin particles. Molecules smaller than the isolation range (35nm+ or 70nm+) are slowed because they enter into the pores of the stationary phase. Larger particles which cannot enter the pores flow around the resin and are eluted from the column earlier. Molecules and small particles that enter the pores have longer retention times and elute later.



How qEV Works



qEV is based on size exclusion chromatography (SEC). Size exclusion chromatography uses a stationary phase consisting of porous resin particles. Molecules smaller than the isolation range (35nm+ or 70nm+) are slowed because they enter into the pores of the stationary phase. Larger particles which cannot enter the pores flow around the resin and are eluted from the column earlier. Molecules and small particles that enter the pores have longer retention times and elute later.




Choose an Exosome Isolation Column Optimised For Your Research.


There are currently 4 different Izon columns sizes to choose from, each available in 2 isolation ranges (designated 35 or 70). As each column has its own advantages, we’ll guide you through the process of choosing the right one for your research. The important selection considerations are sample volume and expected particle sizes. All 8 of our columns benefit from our ISO 13485:2016 certification.


Step 1 – Choose Your Column Size


qEVsingle



qEVoriginal



qEV2



qEV10



  • < 150 µL

    Sample loading (recommended for highest purity).

  • Ideal for clinical samples, RT-PCR

    Optimised for small samples. No RNA carryover.

  • Single Use

    Cost efficient.


  • < 500 µL

    Sample loading (recommended for highest purity).

  • Ideal for higher volume research

    ISO 13485 Certified. The original, and most popular qEV column.

  • Reusable

    Up to 5 times.

  • < 2 ml

    Sample loading (recommended for highest purity).

  • Ideal for larger clinical samples & RNA preparation

    Includes Leur Lock fitting.

  • Reusable

    Up to 5 times.


  • < 10 ml

    Sample loading (recommended for highest purity).

  • Ideal for large volume cell culture supernatant

    Includes Leur Lock fitting.

  • Reusable

    Up to 5 times.


Step 1 – Choose Your Column Size


qEVsingle

  • < 150 µL

    Sample loading (recommended for highest purity).

  • Ideal for clinical samples, RT-PCR

    Optimised for small samples. No RNA carryover.

  • Single Use

    Cost efficient.


qEVsingle


qEVoriginal

  • < 500 µL

    Sample loading (recommended for highest purity).

  • Ideal for higher volume research

    ISO 13485 Certified. The original, and most popular qEV column.

  • Reusable

    Up to 5 times.


qEVoriginal



qEV2

  • < 2 ml

    Sample loading (recommended for highest purity).

  • Ideal for larger clinical samples & RNA preparation

    Includes Leur Lock fitting.

  • Reusable

    Up to 5 times.


qEV2


qEV10

  • < 10 ml

    Sample loading (recommended for highest purity).

  • Ideal for large volume cell culture supernatant

    Includes Leur Lock fitting.

  • Reusable

    Up to 5 times.


qEV10

Step 2 – Choose Your Isolation Range


Each of the four different column sizes is now available in two isolation ranges (35nm+ and 70nm+). The popular 70nm+ qEV columns have an optimum recovery of particles from 70nm to 1000nm, while the newer 35nm+ columns have an optimum recovery range of 35nm to 200nm.




qEV / 35nm


  • 35nm - 200nm

    Optimum Recovery Range

  • <110nm

    Higher recovery of EVs smaller than 110nm

  • More Liposome Overlap

    When working with blood plasma


qEV / 70nm


  • 70nm - 1000nm

    Optimum Recovery Range

  • >110nm

    Higher recovery of EVs larger than 110nm

  • Less Liposome Overlap

    When working with blood plasma

qEV / 35nm


  • 35nm - 200nm

    Optimum Recovery Range

  • <110nm

    Higher recovery of particles smaller than 110nm

  • More Liposome Overlap

    When working with blood plasma



qEV / 70nm


  • 70nm - 1000nm

    Optimum Recovery Range

  • >110nm

    Higher recovery of particles larger than 110nm

  • Less Liposome Overlap

    When working with blood plasma

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