Detailed Studies of Particle Functionalisation Kinetics aid Diagnostics Research

Researchers at the Biomedical Diagnostics Institute at the National Centre for Sensor Research at Dublin City University have chosen Izon’s qNano system to advance their research into functionalised nanoparticles for diagnostic applications. One of the projects is to develop an assay using antibody functionalised nanoparticles to accurately detect and measure antigens of interest. Detailed engineering of the system requires optimisation of many factors including particle size and charge, antibody-particle ratio, checking the rate of the antibody-antigen reactions, and using the kinetics of agglutionation to determine antigen quantity. Accurately quantifying the number of particles and biomolecules being bound is an essential step in the engineering process.

“The qNano’s single-particle detection approach, and unique resolution capability in both particle size and surface charge, provide valuable insight into the sensitisation process of nanoparticles for bio-diagnostic applications.”

Key researcher on the project, Dr. Vladimir Gubala, said he is excited about the initial results and sees the qNano being applied across many research projects within the group.”Izon has provided us with so much support on this work and really made it easy for us to push the limits and try new ideas” he said. “We’re doing research that is really taking the platform to a new place and showing what it can do, and we’re excited to be the ones to do it.”

Further reading: A comparison of mono and multivalent linkers and their effect on the colloidal stability of nanoparticle and immunoassays performance. Gubala V. Le Guevel X. Nooney R. Williams DE. MacCraith B. Talanta. 81(4-5):1833-9, 2010 Jun 15.